粉防己碱对提高人鼻咽癌细胞放射敏感性的作用

[Effects of tetrandrine on enhancing radiosensitivity of human nasopharyngeal carcinoma cells].

作者信息

Wang Kai, Zhang Gehua, Chang Lihong, Wu Xifu, Li Jingjia, Zhuang Shimin, Wang Jun, Daqing Li

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, Third Affiliated Hospital of SUN Yat-sen University, Guangzhou 510630, China.

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出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Mar;49(3):227-31.

PMID:24820495

Abstract

OBJECTIVE

To study the effect and mechanism of tetrandrine (Tet) on enhancing radiosensitivity of human nasopharyngeal carcinoma cell lines in vitro.

METHODS

CNE1 and CNE2 were exposed to radiation with or without Tet, the DNA damage of the cells were evaluated by neutral comet electrophoresis, and cell cycle and apoptosis were analyzed by flow cytometry.

RESULTS

The mean tail movements (TM) of CNE1 treated with radiation or radiation plus Tet were (7.13 ± 3.70) (X(-) ± s) and (13.61 ± 5.45), respectively (t = 2.784, P < 0.05), and TM of CNE2 treated with radiation or radiation plus Tet were (11.52 ± 4.04) and (18.85 ± 6.18), respectively (t = 3.089, P < 0.05). With the exposure to radiation or radiation plus Tet, the percentages of CNE1 in G2 phases were (42.62 ± 2.07)% and (17.02 ± 1.87)%, respectively (t = 23.173, P < 0.01), and the percentages of CNE2 in G2 phases were (34.82 ± 2.74)% and (19.64 ± 4.82)%, respectively(t = 16.500, P < 0.01). There was no significant difference in the apoptosis rates between the cells treated with radiation or radiation plus Tet regardless of CNE1 (17.24 ± 0.99)% vs (19.11 ± 1.24)%, and CNE2 (16.68 ± 0.27)% vs (18.51 ± 2.41)% (P > 0.05).

CONCLUSIONS

Tet can enhance radiosensitivity of human nasopharyngeal carcinoma cell lines. The mechanism could be related to abrogation of radiation-induced G2/M arrest and reduction of double-strand break repair capacity.

摘要

目的

研究粉防己碱（Tet）体外增强人鼻咽癌细胞系放射敏感性的作用及机制。

方法

将CNE1和CNE2细胞分别进行有或无Tet处理后接受辐射，通过中性彗星电泳评估细胞的DNA损伤，采用流式细胞术分析细胞周期和凋亡情况。

结果

单纯辐射处理的CNE1细胞平均彗尾迁移率（TM）为（7.13±3.70）（X(-)±s），辐射加Tet处理的为（13.61±5.45），差异有统计学意义（t = 2.784，P < 0.05）；单纯辐射处理的CNE2细胞TM为（11.52±4.04），辐射加Tet处理的为（18.85±6.18），差异有统计学意义（t = 3.089，P < 0.05）。接受辐射或辐射加Tet处理后，CNE1细胞G2期比例分别为（42.62±2.07）%和（17.02±1.87）%，差异有极显著统计学意义（t = 23.173，P < 0.01）；CNE2细胞G2期比例分别为（34.82±2.7）%和（19.64±4.82）%，差异有极显著统计学意义（t = 16.500，P < 0.01）。无论CNE1（17.24±0.99）%与（19.还是CNE2（16.68±0.27）%与（18.51±2.41）%，单纯辐射处理组与辐射加Tet处理组细胞凋亡率差异均无统计学意义（P > 0.05）。