Laporte Martin, Shao Zhaojun, Berrebi Patrick, Laabir Mohamed, Abadie Eric, Faivre Nicolas, Rieuvilleneuve Fabien, Masseret Estelle
Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France; Département de Biologie, IBIS (Institut de Biologie Intégrative et des Systèmes), Université Laval, Québec G1V 0A6, Canada.
Institut des Sciences de l'Evolution de Montpellier, UMR 5554 UM2-CNRS-IRD, Université Montpellier II, CC065, Place E. Bataillon, 34095 Montpellier, Cedex 5, France.
Mar Pollut Bull. 2014 Jun 15;83(1):302-5. doi: 10.1016/j.marpolbul.2014.04.017. Epub 2014 May 10.
Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic species. Finally, the haplotypic diversity ranged from 0.000 to 0.791 and 0.000 to 0.942 for A. catenella and A. tamarense respectively.
链状亚历山大藻(第四组)和塔玛亚历山大藻(第三组)(甲藻纲)是塔玛亚历山大藻复合种中的两种隐性入侵浮游植物物种。它们在全球范围内的传播因人类活动、运输和气候变化而得到促进。为了描述它们在地中海的多样性,并了解它们在该地区的定居和维持情况,基于法国托湖的链状亚历山大藻和塔玛亚历山大藻菌株样本开发了新的微卫星标记。在本研究中,提出了12个新的微卫星标记。其中5个微卫星标记在塔玛亚历山大藻上显示出扩增,10个在链状亚历山大藻上显示出扩增。这12个微卫星标记中的3个在这两种隐性物种上都能扩增。最后,链状亚历山大藻和塔玛亚历山大藻的单倍型多样性分别为0.000至0.791和0.000至0.942。