远缘链球菌6715表面蛋白抗原基因spaA1决定簇的遗传特征分析

Genetic characterization of the spaA1 determinant of the surface protein antigen gene from Streptococcus sobrinus 6715.

Saito S, Abiko Y, Koyanagi T, Yazawa Y, Takiguchi H

Department of Biochemistry, Nihon University School of Dentistry, Matsudo, Japan.

Int J Biochem. 1989;21(11):1245-54. doi: 10.1016/0020-711x(89)90011-6.

Abstract

The surface protein antigen-encoding gene spaA1, previously cloned from Streptococcus sobrinus 6715 (serotype g) into the plasmid pACYC184, was examined. 2. The gene product of pYA726 was identified both by the minicell method and by in vitro transcription-translation system as a protein whose mol. wt was estimated to be 140,000. 3. The spaA1 gene was localized on the 3.75 kb AvaI-BamHI fragment of the cloned DNA. 4. An internal spaA1 promoter located close to one end of the 1.25 kb AvaI fragment within the 3.75 kb AvaI-BamHI fragment initiated the transcription for the insert DNA of S. sobrinus autonomously.

摘要

对先前从远缘链球菌6715（血清型g）克隆到质粒pACYC184中的表面蛋白抗原编码基因spaA1进行了检测。2. 通过小细胞法和体外转录-翻译系统鉴定出pYA726的基因产物是一种分子量估计为140,000的蛋白质。3. spaA1基因定位在克隆DNA的3.75 kb AvaI - BamHI片段上。4. 位于3.75 kb AvaI - BamHI片段内1.25 kb AvaI片段一端附近的一个内部spaA1启动子自主启动了远缘链球菌插入DNA的转录。