草本适应原与牛初乳和鸡蛋黄中的蛋白质片段结合使用可降低西方饮食喂养大鼠肝脏 TNF-α 表达和蛋白质羰基化。

Herbal adaptogens combined with protein fractions from bovine colostrum and hen egg yolk reduce liver TNF-α expression and protein carbonylation in Western diet feeding in rats.

机构信息

School of Kinesiology, Auburn University, Auburn, AL, USA.

Department of Biomedical Sciences, University of Missouri, Columbia, MO, USA.

出版信息

Nutr Metab (Lond). 2014 Apr 23;11:19. doi: 10.1186/1743-7075-11-19. eCollection 2014.

DOI:10.1186/1743-7075-11-19

PMID:24822076

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4017828/

Abstract

BACKGROUND

We examined if a purported anti-inflammatory supplement (AF) abrogated Western-diet (WD)-induced liver pathology in rats. AF contained: 1) protein concentrates from bovine colostrum and avian egg yolk; 2) herbal adaptogens and antioxidants; and 3) acetyl-L-carnitine.

METHODS

Nine month-old male Brown Norway rats were allowed ad libitum access to WD for 41-43 days and randomly assigned to WD + AF feeding twice daily for the last 31-33 days (n = 8), or WD and water-placebo feeding twice daily for the last 31-33 days (n = 8). Rats fed a low-fat/low-sucrose diet (CTL, n = 6) for 41-43 days and administered a water-placebo twice daily for the last 31-33 days were also studied. Twenty-four hours following the last gavage-feed, liver samples were analyzed for: a) select mRNAs (via RT-PCR) as well as genome-wide mRNA expression patterns (via RNA-seq); b) lipid deposition; and, c) protein carbonyl and total antioxidant capacity (TAC). Serum was also examined for TAC, 8-isoprostane and clinical chemistry markers.

RESULTS

WD + AF rats experienced a reduction in liver Tnf-α mRNA (-2.8-fold, p < 0.01). Serum and liver TAC was lower in WD + AF versus WD and CTL rats (p < 0.05), likely due to exogenous antioxidant ingredients provided through AF as evidenced by a tendency for mitochondrial SOD2 mRNA to increase in WD + AF versus CTL rats (p = 0.07). Liver fat deposition nor liver protein carbonyl content differed between WD + AF versus WD rats, although liver protein carbonyls tended to be lower in WD + AF versus CTL rats (p = 0.08). RNA-seq revealed that 19 liver mRNAs differed between WD + AF versus WD when both groups were compared with CTL rats (+/- 1.5-fold, p < 0.01). Bioinformatics suggest that AF prevented WD-induced alterations in select genes related to the transport and metabolism of carbohydrates in favor of select genes related to lipid transport and metabolism. Finally, serum clinical safety markers and liver pathology (via lesion counting) suggests that chronic consumption of AF was well tolerated.

CONCLUSIONS

AF supplementation elicits select metabolic, anti-inflammatory, and anti-oxidant properties which was in spite of WD feeding and persisted up to 24 hours after receiving a final dose.

摘要

背景

我们研究了一种据称具有抗炎作用的补充剂（AF）是否能消除西式饮食（WD）诱导的大鼠肝病理。AF 包含：1）牛初乳和禽卵黄的蛋白质浓缩物；2）草药适应原和抗氧化剂；3）乙酰左旋肉碱。

方法

9 月龄雄性棕色挪威大鼠可随意摄入 WD 长达 41-43 天，并在最后 31-33 天内随机分配接受 WD+AF 每日两次喂养（n=8），或 WD 和水安慰剂每日两次喂养（n=8）。还研究了 41-43 天内摄入低脂/低糖饮食（CTL，n=6）并在最后 31-33 天内接受水安慰剂两次每日喂养的大鼠。最后一次灌胃后 24 小时，分析肝样品：a）通过 RT-PCR 分析选定的 mRNAs 以及通过 RNA-seq 分析全基因组 mRNA 表达模式；b）脂质沉积；c）蛋白质羰基和总抗氧化能力（TAC）。还检查了血清中的 TAC、8-异前列腺素和临床化学标志物。

结果

WD+AF 大鼠的肝 TNF-α mRNA 减少了 2.8 倍（p<0.01）。与 WD 和 CTL 大鼠相比，WD+AF 大鼠的血清和肝 TAC 较低（p<0.05），这可能是由于通过 AF 提供了外源性抗氧化成分，这从 WD+AF 大鼠的线粒体 SOD2 mRNA 有增加的趋势（p=0.07）中得到证实。与 WD 大鼠相比，WD+AF 大鼠的肝脂肪沉积或肝蛋白质羰基含量无差异，但 WD+AF 大鼠的肝蛋白质羰基含量有降低的趋势（p=0.08）。RNA-seq 显示，当将 WD+AF 组与 CTL 组进行比较时，19 种肝 mRNA 在 WD+AF 组与 WD 组之间存在差异（+/-1.5 倍，p<0.01）。生物信息学表明，AF 阻止了 WD 诱导的与碳水化合物转运和代谢相关的某些基因的改变，有利于与脂质转运和代谢相关的某些基因。最后，血清临床安全性标志物和肝病理（通过病变计数）表明，AF 的慢性消耗是可以耐受的。