半薄切片在软性下疳诊断中的应用

Utility of semithin sections in the diagnosis of donovanosis.

作者信息

Jain A K, Chandra M, Ganguli D D, Bhargava N C

出版信息

Indian J Med Res. 1989 Aug;90:270-4.

Abstract

The relative merits of tissue smears, paraffin sections and semithin plastic embedded sections in the diagnosis of donovanosis were assessed. In three cases reported negative or doubtful for the organisms by the former two methods, semithin plastic sections provided definite diagnosis. The latter method revealed in addition the organisms in epidermis and perivascular locations. From amongst the three stains: toluidine blue, Leishman's and thionine azure II basic fuchsin, the last mentioned was found to be the method of choice for demonstrating the organisms differently from the host cells.

摘要

评估了组织涂片、石蜡切片和半薄塑料包埋切片在诊断杜诺凡病中的相对优点。在前两种方法报告为该病原体阴性或可疑的3例病例中，半薄塑料切片提供了明确的诊断。后一种方法还揭示了表皮和血管周围部位的病原体。在甲苯胺蓝、利什曼染色和硫堇天蓝II碱性品红这三种染色方法中，发现最后提到的方法是区分病原体与宿主细胞的首选方法。