六氯环己烷（HCH）对大鼠离体输精管中去甲肾上腺素诱导的收缩反应的影响。

Effects of hexachlorocyclohexane (HCH) on the contractile response induced by norepinephrine in the rat isolated vas deferens.

作者信息

Gandhi D N, Venkatakrishna-Bhatt H

机构信息

Department of Neurobehavioural Toxicology, National Institute of Occupational Health (ICMR), Gujarat, India.

出版信息

Pharmacol Toxicol. 1989 Nov;65(5):376-80. doi: 10.1111/j.1600-0773.1989.tb01192.x.

DOI:10.1111/j.1600-0773.1989.tb01192.x

PMID:2482969

Abstract

Effects of hexachlorocyclohexane (Technical HCH) on the contractile responses of rat isolated Vas deferens to norepinephrine (NE) were investigated by varying the Ca++ and Mg++ concentrations of the incubation medium. Preparations incubated in excess Ca++ (4.2 mM) or in Ca++-free Krebs solution showed decrease in sensitivity to NE. The inhibitory action of HCH reduced the sensitivity and decreased maximum responses to NE in any Mg++ concentration of the medium. In the normal Ca++ and its deficient medium decrease in sensitivity to HCH and the relevant maximum response to NE were observed. In excess Ca++, the HCH effect depressed corresponding to its maximal response to NE. The presence of EDTA depressed the HCH effect irrespective of the Ca++ influence on the receptors.

摘要

通过改变孵育介质中钙离子（Ca++）和镁离子（Mg++）的浓度，研究了六氯环己烷（工业用六氯环己烷）对大鼠离体输精管对去甲肾上腺素（NE）收缩反应的影响。在过量钙离子（4.2 mM）中孵育的制剂或在无钙离子的克雷布斯溶液中孵育的制剂对去甲肾上腺素的敏感性降低。在介质的任何镁离子浓度下，六氯环己烷的抑制作用都会降低对去甲肾上腺素的敏感性并减少其最大反应。在正常钙离子及其缺乏的介质中，观察到对六氯环己烷的敏感性降低以及对去甲肾上腺素的相应最大反应降低。在过量钙离子存在下，六氯环己烷的作用与其对去甲肾上腺素的最大反应相对应而降低。乙二胺四乙酸（EDTA）的存在会抑制六氯环己烷的作用，而与钙离子对受体的影响无关。