Rahbar Kafshboran Haniyeh, Bonyadi Mortaza, Miri Hamidreza, Haghi Mehdi, Nikravesh Abbas, Abdolmohammadi Reza, Hossein Somi Mohammad, Khoshbaten Manouchehr
Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran ; Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran ; Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Middle East J Dig Dis. 2014 Jan;6(1):28-31.
BACKGROUND Inflammatory bowel disease (IBD) is a chronic, relapsing, inflammatory disorder of the gastrointestinal tract that includes two entities, Crohn's disease (CD) and ulcerative colitis (UC). As with other complex diseases, both genetic susceptibility and environmental factors play role in the pathogenesis of these diseases. The tumor necrosis factor α (TNF-α) gene is located in the IBD3 region on chromosome 6p21 which is a good functional candidate for involvement in susceptibility to IBD. In addition, the promoter region of TNF-α contains various polymorphisms that have shown a significant association with IBD. METHODS In this case control study we investigated the TNF-α -857 polymorphism in 109 patients (89 UC and 16 CD) who suffered from IBD and 100 healthy age, sex and ethnicity matched adults selected from the same population, as the control group. The polymorphism was checked by amplification refractory system (ARMS) and polymerase chain reaction (PCR). RESULTS Investigation of the association of TNF-α -857 gene promoter polymorphism with both types of IBD showed no significant difference in genotype and allele frequencies of this polymorphism between UC patients and controls. However, a possible association of TNF-α -857 polymorphism (p=0.03) was identified with CD. CONCLUSION TNF-α -857 polymorphism may have a role in the development of CD in the Iranian Azeri Turkish population.
炎症性肠病（IBD）是一种胃肠道的慢性、复发性炎症性疾病，包括克罗恩病（CD）和溃疡性结肠炎（UC）两个类型。与其他复杂疾病一样，遗传易感性和环境因素在这些疾病的发病机制中均起作用。肿瘤坏死因子α（TNF-α）基因位于6号染色体p21的IBD3区域，是参与IBD易感性的一个良好功能候选基因。此外，TNF-α的启动子区域包含多种多态性，这些多态性已显示与IBD存在显著关联。
在这项病例对照研究中，我们调查了109例IBD患者（89例UC和16例CD）以及100名从同一人群中选取的年龄、性别和种族匹配的健康成年人（作为对照组）的TNF-α -857多态性。通过扩增阻滞突变系统（ARMS）和聚合酶链反应（PCR）检测该多态性。
对TNF-α -857基因启动子多态性与两种类型IBD的关联性研究表明，UC患者与对照组之间该多态性的基因型和等位基因频率无显著差异。然而，发现TNF-α -857多态性与CD可能存在关联（p = 0.03）。
TNF-α -857多态性可能在伊朗阿塞拜疆土耳其人群的CD发病中起作用。
