Jackson Alexis M, Semmens Brice X, Sadovy de Mitcheson Yvonne, Nemeth Richard S, Heppell Scott A, Bush Phillippe G, Aguilar-Perera Alfonso, Claydon John A B, Calosso Marta C, Sealey Kathleen S, Schärer Michelle T, Bernardi Giacomo
Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, California, United States of America.
Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, United States of America.
PLoS One. 2014 May 15;9(5):e97508. doi: 10.1371/journal.pone.0097508. eCollection 2014.
To address patterns of genetic connectivity in a mass-aggregating marine fish, we analyzed genetic variation in mitochondrial DNA (mtDNA), microsatellites, and single nucleotide polymorphisms (SNPs) for Nassau grouper (Epinephelus striatus). We expected Nassau grouper to exhibit genetic differentiation among its subpopulations due to its reproductive behavior and retentive oceanographic conditions experienced across the Caribbean basin. All samples were genotyped for two mitochondrial markers and 9 microsatellite loci, and a subset of samples were genotyped for 4,234 SNPs. We found evidence of genetic differentiation in a Caribbean-wide study of this mass-aggregating marine fish using mtDNA (FST = 0.206, p<0.001), microsatellites (FST = 0.002, p = 0.004) and SNPs (FST = 0.002, p = 0.014), and identified three potential barriers to larval dispersal. Genetically isolated regions identified in our work mirror those seen for other invertebrate and fish species in the Caribbean basin. Oceanographic regimes in the Caribbean may largely explain patterns of genetic differentiation among Nassau grouper subpopulations. Regional patterns observed warrant standardization of fisheries management and conservation initiatives among countries within genetically isolated regions.
为了研究一种大规模聚集的海洋鱼类的遗传连通性模式，我们分析了拿骚石斑鱼（Epinephelus striatus）线粒体DNA（mtDNA）、微卫星和单核苷酸多态性（SNP）的遗传变异。由于其繁殖行为以及整个加勒比海盆地所经历的滞留海洋学条件，我们预计拿骚石斑鱼的亚种群之间会表现出遗传分化。所有样本针对两个线粒体标记和9个微卫星位点进行了基因分型，一部分样本针对4234个SNP进行了基因分型。我们在一项针对这种大规模聚集海洋鱼类的全加勒比海研究中发现了遗传分化的证据，该研究使用了mtDNA（FST = 0.206，p < 0.001）、微卫星（FST = 0.002，p = 0.004）和SNP（FST = 0.002，p = 0.014），并确定了三个潜在的幼体扩散障碍。我们研究中确定的基因隔离区域与加勒比海盆地其他无脊椎动物和鱼类物种的情况相似。加勒比海的海洋学状况可能在很大程度上解释了拿骚石斑鱼亚种群之间的遗传分化模式。观察到的区域模式需要在基因隔离区域内的国家之间对渔业管理和保护举措进行标准化。
