三维细胞培养系统及其在药物发现和基于细胞的生物传感器中的应用。
Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors.
作者信息
Edmondson Rasheena, Broglie Jessica Jenkins, Adcock Audrey F, Yang Liju
机构信息
Biomanufacturing Research Institute and Technology Enterprises (BRITE), and Department of Pharmaceutical Sciences, North Carolina Central University , Durham, North Carolina.
出版信息
Assay Drug Dev Technol. 2014 May;12(4):207-18. doi: 10.1089/adt.2014.573.
Abstract
Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests. In this review, we discuss the characteristics of 3D cell culture systems in comparison to the two-dimensional (2D) monolayer culture, focusing on cell growth conditions, cell proliferation, population, and gene and protein expression profiles. The innovations and development in 3D culture systems for drug discovery over the past 5 years are also reviewed in the article, emphasizing the cellular response to different classes of anticancer drugs, focusing particularly on similarities and differences between 3D and 2D models across the field. The progression and advancement in the application of 3D cell cultures in cell-based biosensors is another focal point of this review.
摘要
三维（3D）细胞培养系统在药物发现和组织工程领域越来越受到关注，因为它们在为体内试验提供更多生理相关信息和更具预测性的数据方面具有明显优势。在这篇综述中，我们讨论了三维细胞培养系统与二维（2D）单层培养相比的特点，重点关注细胞生长条件、细胞增殖、群体以及基因和蛋白质表达谱。本文还回顾了过去5年用于药物发现的三维培养系统的创新和发展，强调细胞对不同类抗癌药物的反应，特别关注该领域三维和二维模型之间的异同。三维细胞培养在基于细胞的生物传感器中的应用进展是本综述的另一个重点。
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