Sugiura Y, Araie M
Department of Ophthalmology, University of Tokyo School of Medicine.
Jpn J Ophthalmol. 1989;33(4):441-50.
In an attempt to study the effect of change in the intraocular pressure (IOP) on the status of the vitreous-aqueous interface, the effect of topical bunazosin was studied in the rabbit eye using the new Johnson-Maurice method. This entails an intravitreal injection of FITC-dextran and measurements of its concentration changes in the anterior chamber many days after the injection. Repeated instillations of bunazosin solution reduced the IOP by about 5 mmHg, without changing the aqueous flow rate as determined by the method of fluorometry. On the other hand, the FITC-dextran concentration in the anterior chamber increased, indicating that the movement of FITC-dextran from the vitreous into the aqueous was greater when the IOP was lowered. This increase in the FITC-dextran movement into the anterior chamber was thought to be mainly attributable to an increase in the anteriorly directed fluid movement across the vitreous-aqueous interface. The increase in the fluid movement was estimated to be about 1.3 microliter/hour. This study suggested that a reduction in the IOP generated a small fluid flow from the vitreous into the aqueous, and that the new Johnson-Maurice method can be a useful means for noninvasive investigations of the physiology of the vitreous-aqueous interface.
为了研究眼内压（IOP）变化对玻璃体-房水界面状态的影响，采用新的约翰逊-莫里斯方法在兔眼中研究了局部使用布那唑嗪的效果。这需要玻璃体腔内注射异硫氰酸荧光素标记的葡聚糖，并在注射数天后测量其在前房中的浓度变化。反复滴注布那唑嗪溶液可使眼内压降低约5 mmHg，而通过荧光测定法测定的房水流量未发生变化。另一方面，前房中的异硫氰酸荧光素标记的葡聚糖浓度增加，表明当眼内压降低时，异硫氰酸荧光素标记的葡聚糖从玻璃体进入房水的移动更大。异硫氰酸荧光素标记的葡聚糖向房水移动的这种增加被认为主要归因于穿过玻璃体-房水界面的向前液体移动增加。液体移动的增加估计约为1.3微升/小时。这项研究表明，眼内压降低会产生从玻璃体到房水的少量液体流动，并且新的约翰逊-莫里斯方法可以成为无创研究玻璃体-房水界面生理学的有用手段。
