HepG2.2.15 作为研究 S100 蛋白调控细胞突出和迁移的模型。
HepG2.2.15 as a model for studying cell protrusion and migration regulated by S100 proteins.
机构信息
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, PR China.
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, PR China.
出版信息
Biochem Biophys Res Commun. 2014 Jun 20;449(1):175-81. doi: 10.1016/j.bbrc.2014.05.010. Epub 2014 May 14.
Much of the difficulty in elucidating the precise function of S100 protein family has been attributed to functional redundancy and compensation by its conserved family members. In this study, we showed that seven S100 family members were almost totally undetectable in HepG2.2.15 cells, while all of them were highly expressed in its parental HepG2 cells. Re-expression of S100 proteins in HepG2.2.15 cells can partially rescue their defects in cell protrusion and migration through the regulation of cytoskeletons and adhesions. Thus, HepG2.2.15 can serve as a useful model for studying cell protrusion and migration regulated by S100 proteins.
在阐明 S100 蛋白家族的确切功能方面存在诸多困难，其主要原因是其保守家族成员存在功能冗余和补偿。在本研究中，我们发现 HepG2.2.15 细胞中几乎完全检测不到七种 S100 家族成员，而在其亲本 HepG2 细胞中，它们都高度表达。在 HepG2.2.15 细胞中重新表达 S100 蛋白可以部分通过调节细胞骨架和黏附物来挽救其在细胞突起和迁移方面的缺陷。因此，HepG2.2.15 可作为研究 S100 蛋白调控的细胞突起和迁移的有用模型。
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