抗弯曲杆菌鞭毛蛋白的抗体识别1期和2期鞭毛共有的表位。

Antibodies to Campylobacter flagellin recognize epitopes common to phase 1 and phase 2 flagella.

作者信息

Kirimat M, Georges-Courbot M C, Georges A J, Martin P M

机构信息

Institut Pasteur, Bangui.

出版信息

Res Microbiol. 1989 Nov-Dec;140(9):645-51. doi: 10.1016/0923-2508(89)90196-4.

DOI:10.1016/0923-2508(89)90196-4

PMID:2483275

Abstract

We used a simple method to obtain purified flagellin from Campylobacter, suitable for an immunization procedure in mice. Western blot analysis of cross-reacting antibodies showed that there were epitopes common to phase 1 and 2 flagellins. Analysis by ELISA suggested that certain common flagellar epitopes are conformational, and antibody immobilization tests confirmed that common surface-exposed epitopes exist in a region of flagella necessary for conferring motility to the bacteria.

摘要

我们采用一种简单的方法从弯曲杆菌中获得纯化的鞭毛蛋白，该方法适用于小鼠免疫程序。对交叉反应抗体的蛋白质免疫印迹分析表明，1期和2期鞭毛蛋白存在共同表位。酶联免疫吸附测定分析表明某些共同的鞭毛表位是构象性的，抗体固定试验证实，共同的表面暴露表位存在于鞭毛的一个区域，该区域对于赋予细菌运动性是必需的。