Sukhikh T R, Kalimagambetov A M, Barskiĭ I Ia, Khomenkova S A, Vinogradov A E
Tsitologiia. 1989 Nov;31(11):1329-38.
A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.
基于两步分析法，已制定出一种测定人类个体染色体中DNA含量的方法：1）通过Q带鉴定染色体；2）对福尔根染色后的染色体进行显微荧光测定（一种使用金胺-SO2席夫型试剂的荧光变体）。以绝对值（fg）计算了24条人类染色体中的DNA含量。将获得的数据与其他地方发表的证据以及不同细胞光度法提供的证据进行了比较。
