Madewell B R, Gumerlock P H, Saunders K A, Meyers F J, Kraegel S A, Kawasaki E S
Department of Veterinary Surgery, University of California School of Veterinary Medicine, Davis 95616.
Anticancer Res. 1989 Nov-Dec;9(6):1743-9.
Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
细胞原癌基因是高度保守的基因，被认为在细胞生长和分化中起关键作用。在本研究中，我们使用人源序列设计的寡核苷酸引物，通过对基因组DNA进行聚合酶链反应（PCR）扩增（DNA/PCR）来检测和区分犬和牛的c-Ha-ras-1、c-Ki-ras-2和c-N-ras基因。此外，我们还应用PCR对从RAS基因转录的表达mRNA进行分析（RNA/PCR）。
