Maeda Y, Yoneda K, Osaki T
Department of Oral and Maxillofacial Surgery, Kochi Medical School, Nankoku-city, Japan.
Basic Appl Histochem. 1989;33(4):311-23.
Lectin-binding patterns were examined in epithelial walls of 65 jaw cysts (30 post-operative maxillary cysts: POMCs, 20 radicular and 15 follicular cysts), and characteristic lectin staining for each kind of jaw cysts is presented. Between squamous and columnar epithelia, the staining intensity of WGA, Con A and UEA-I was not different, but SBA bound more remarkably to squamous than to columnar epithelia. In both epithelia the outer layers did react more strongly with the lectins examined. Concerning odontogenic cysts, the lectin-binding affinities of outer and intermediate layer cells were nearly the same in both follicular and radicular cysts. Basal cells of radicular cyst walls were however, more markedly positive for lectin binding than of follicular cysts. Furthermore, basal cells of keratinized (RKSE 60 keratin-positive) epithelium were inferior to those of non-keratinized linings in the bindings. Lectin-binding patterns of metaplastic squamose epithelia of POMCs which were positive for RGE53-keratin (principally columnar epithelium-specific keratin) were similar to originally squamous linings of odontogenic cysts. Columnar linings of unusual radicular cysts were positively stained with SBA. By these results, lectin-binding sugar residues of the epithelium seem to be related to the epithelial morphology.
研究了65个颌骨囊肿（30个术后上颌囊肿：POMC，20个根端囊肿和15个滤泡囊肿）上皮壁中的凝集素结合模式，并展示了每种颌骨囊肿的特征性凝集素染色。在鳞状上皮和柱状上皮之间，WGA、Con A和UEA-I的染色强度没有差异，但SBA与鳞状上皮的结合比与柱状上皮更显著。在两种上皮中，外层与所检测的凝集素反应更强。关于牙源性囊肿，在滤泡囊肿和根端囊肿中，外层和中间层细胞的凝集素结合亲和力几乎相同。然而，根端囊肿壁的基底细胞比滤泡囊肿的基底细胞在凝集素结合方面更明显呈阳性。此外，角化（RKSE 60角蛋白阳性）上皮的基底细胞在结合方面不如非角化衬里的基底细胞。对RGE53角蛋白（主要是柱状上皮特异性角蛋白）呈阳性的POMC化生鳞状上皮的凝集素结合模式与牙源性囊肿原来的鳞状衬里相似。异常根端囊肿的柱状衬里被SBA阳性染色。根据这些结果，上皮的凝集素结合糖残基似乎与上皮形态有关。
