Ginther O J, Rakesh H B, Hoffman M M
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Eutheria Foundation, Cross Plains, Wisconsin, USA.
Theriogenology. 2014 Jul 15;82(2):304-11. doi: 10.1016/j.theriogenology.2014.04.009. Epub 2014 Apr 16.
The hemodynamics of the developing CL and the future dominant follicle (DF) was studied in 22 heifers during wave 1 on Days 0 to 5 (Day 0 = ovulation). Color-Doppler ultrasonography was used to determine the resistance index (RI) at the most prominent Doppler signal in an ovarian arterial branch before entry into the ovary; a decrease in RI indicates a downstream increase in vascular perfusion. The RI for each of four intraovarian patterns averaged over days was different (P < 0.05) from each of the other patterns as follows: DF-CL (DF and CL in the same ovary), 0.52 ± 0.02; CL alone, 0.60 ± 0.01; DF alone, 0.67 ± 0.01; neither DF nor CL, 0.78 ± 0.01. The differences in RI among intraovarian patterns began on Day 0 or 1, indicating that the extent of vascular perfusion on Days 0 to 5 for the various patterns may have been influenced by events that occurred before ovulation. The percentage of the DF wall with color-flow signals was greater (P < 0.05) in the DF-CL pattern than in the DF pattern on each of Days 2 to 5 and was greater (P < 0.0001) in the DF-CL pattern when the DF was adjacent to the CL (40.2 ± 2.0%) than when separated (24.5 ± 1.9%). Dimensions of DF (P < 0.01) and CL (P < 0.02) were greater when adjacent to each other. The results supported the hypotheses for wave 1 that (1) vascular perfusion is greater for the DF-CL intraovarian pattern than for the DF or CL pattern and (2) the extent of blood-flow Doppler signals in the wall of the developing DF is greater for the DF-CL pattern than for the DF pattern. Our preferred interpretation is that a change in vascular perfusion of the CL is accompanied by a similar change in perfusion of the DF when the two structures are in the same ovary especially adjacent.
在第0至5天的第1个卵泡波期间，对22头小母牛发育中的黄体（CL）和未来的优势卵泡（DF）的血流动力学进行了研究（第0天=排卵）。彩色多普勒超声用于确定卵巢动脉分支进入卵巢之前最明显的多普勒信号处的阻力指数（RI）；RI降低表明血管灌注下游增加。四种卵巢内模式各自在数天内的平均RI彼此不同（P<0.05），如下所示：DF-CL（DF和CL在同一卵巢中），0.52±0.02；单独的CL，0.60±0.01；单独的DF，0.67±0.01；既无DF也无CL，0.78±0.01。卵巢内模式之间的RI差异在第0天或第1天开始，表明第0至5天不同模式的血管灌注程度可能受到排卵前发生事件的影响。在第2至5天的每一天，DF-CL模式中具有彩色血流信号的DF壁百分比均高于DF模式（P<0.05），并且当DF与CL相邻时，DF-CL模式中的该百分比更高（P<0.0001）（40.2±2.0%），而当它们分开时为（24.5±1.9%）。当DF和CL彼此相邻时，DF（P<0.01）和CL（P<0.02）的尺寸更大。结果支持了第1个卵泡波的假设，即（1）DF-CL卵巢内模式的血管灌注大于DF或CL模式，（2）发育中的DF壁内血流多普勒信号的程度在DF-CL模式中大于DF模式。我们倾向的解释是，当两个结构在同一卵巢中尤其是相邻时，CL的血管灌注变化伴随着DF灌注的类似变化。
