Combarnous Y, Huet J C, Martinat N, Mansion M, Anouassi A, Pernollet J C
Unité Endocrinologie de la Gamètogenèse, Institut National de la Recherche Agronomique, URA CNRS 234, Nouzilly, France.
Pathol Biol (Paris). 1989 Sep;37(7):814-8.
The subunits of luteinizing hormone from the Camelus dromedarius (CamLH) have been separated by reverse phase HPLC after reduction and alkylation of their disulfide bridges. The N-terminal amino-acid sequencing of the alpha and beta subunits has been performed up to the 53rd and 67th residue respectively (i.e. more than half of each polypeptide chain). These sequences have been compared to those of LH from other mammalian species in order to estimate the phylogenetic divergence of LH in this species and in order to point out characteristic features of its primary structure that can be related to its physico-chemical properties.
来自单峰骆驼的促黄体激素亚基（CamLH）在其二硫键还原和烷基化后，通过反相高效液相色谱法进行了分离。已分别对α和β亚基的N端氨基酸进行测序，直至第53个和第67个残基（即每条多肽链的一半以上）。已将这些序列与其他哺乳动物物种的促黄体激素序列进行比较，以估计该物种促黄体激素的系统发育差异，并指出其一级结构中可能与其物理化学性质相关的特征。
