Egert J, Apitzsch L, Triquart B, Sinnecker H
Central Institute of Hygiene, Microbiology and Epidemiology, Institute of Viral Zoonosis, Potsdam, G.D.R.
Acta Virol. 1989 Dec;33(6):553-8.
The Pasteur strain of fixed rabies virus (Pasteur Institute Paris, passage 2061 in rabbit brain) was adapted by alternate passages to primary dog kidney cells. The adapted rabies virus designated as "Pasteur Potsdam" developed no CPE and yielded four harvests with a titre of 5.5-7.0 (log MICLD50/ml). The strain could be grown in BHK 21/S13, CER and N2a neuroblastoma cells. In the cultures of BHK 21/S13 cells the virus titered 6.0-8.5 (log MICLD50/ml). In SDS PAGE its G protein migrated faster than that of the ERA strain. The inactivated antigen induced interferon in mice. The strain was identified by anti-rabies immunoglobulin. The harvested material showed an antigenic value of 0.4 IU/ml. The virus was not pathogenic after s.c. and i.p. inoculations to mice, rats, Syrian hamsters, and rabbits and after i.m. inoculation to Syrian hamsters and rats.
固定狂犬病病毒的巴斯德株（巴黎巴斯德研究所，在兔脑传代2061次）通过交替传代适应原代犬肾细胞。适应后的狂犬病病毒命名为“巴斯德波茨坦株”，未产生细胞病变效应，收获了四代，滴度为5.5 - 7.0（对数半数感染量致死剂量/毫升）。该毒株可在BHK 21/S13、CER和N2a神经母细胞瘤细胞中生长。在BHK 21/S13细胞培养物中，病毒滴度为6.0 - 8.5（对数半数感染量致死剂量/毫升）。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中，其G蛋白迁移速度比ERA株的G蛋白快。灭活抗原可在小鼠体内诱导干扰素。该毒株通过抗狂犬病免疫球蛋白进行鉴定。收获的材料抗原值为每毫升0.4国际单位。该病毒经皮下和腹腔接种小鼠、大鼠、叙利亚仓鼠和兔子以及肌肉接种叙利亚仓鼠和大鼠后均无致病性。
