Nakagomi T, Ide K, Yamakawa K, Sasaki T, Kurihara H, Saito I, Takakura K
Neurol Med Chir (Tokyo). 1989 Nov;29(11):967-74. doi: 10.2176/nmc.29.967.
The purpose of this study was to evaluate the constrictive effect of endothelin, a peptide vasoconstrictor derived from endothelium, on canine basilar artery. Constriction was measured by an isometric tension recording method. Endothelin induced prolonged contraction of canine basilar artery in a dose-dependent fashion, the estimated concentration at 50% contraction being (2.1 +/- 0.5) X 10(-9) M (mean +/- SEM). Removal of endothelium significantly augmented the arterial response to endothelin. In Ca(++)-free medium no contractile response was elicited at 10(-8) M endothelin. Papaverine (10(-4) M) and nicardipine (10(-8) M), a calcium channel blocker, almost completely inhibited the contraction induced by 10(-8) M endothelin. Pretreatment with nicardipine (10(-8) - 10(-6) M) also significantly suppressed the response to endothelin. Acetylcholine (10(-7) - 10(-4) M), adenosine triphosphate (10(-7) - 10(-5) M), and thrombin (0.1 and 1.0 U/ml) dose-dependently inhibited contraction of canine basilar artery in response to 3 X 10(-9) M endothelin. These results show that endothelin has a potent constrictive effect on canine basilar artery and suggest that this substance may play an important role in the pathogenesis of vasospasm following subarachnoid hemorrhage.
本研究的目的是评估内皮素（一种源自内皮细胞的肽类血管收缩剂）对犬基底动脉的收缩作用。采用等长张力记录法测量收缩情况。内皮素以剂量依赖的方式引起犬基底动脉的长时间收缩，50%收缩时的估计浓度为(2.1±0.5)×10(-9)M（平均值±标准误）。去除内皮显著增强了动脉对内皮素的反应。在无钙培养基中，10(-8)M内皮素未引起收缩反应。罂粟碱(10(-4)M)和钙通道阻滞剂尼卡地平(10(-8)M)几乎完全抑制了10(-8)M内皮素诱导的收缩。用尼卡地平(10(-8)-10(-6)M)预处理也显著抑制了对内皮素的反应。乙酰胆碱(10(-7)-10(-4)M)、三磷酸腺苷(10(-7)-10(-5)M)和凝血酶(0.1和1.0U/ml)以剂量依赖的方式抑制犬基底动脉对3×10(-9)M内皮素的收缩反应。这些结果表明，内皮素对犬基底动脉有强大的收缩作用，并提示该物质可能在蛛网膜下腔出血后血管痉挛的发病机制中起重要作用。
