Rasulov A S, Evstigneeva Z G
Prikl Biokhim Mikrobiol. 1978 Jan-Feb;14(1):150-1.
Highly purified glutamine synthetase has been isolated from Chlorella and immobilized on BrCN-sepharose. Its residual activity was 25-35%. Immobilized glutamine synthetase showed far greater thermal stability than glutamine synthetase in solution. During immobilization pH optimum of the enzyme was shifted towards the alkaline area, maximum rate of the reaction was reduced and KM remained unaltered.
已从小球藻中分离出高度纯化的谷氨酰胺合成酶，并将其固定在溴化氰-琼脂糖上。其残余活性为25%-35%。固定化谷氨酰胺合成酶比溶液中的谷氨酰胺合成酶表现出更高的热稳定性。在固定化过程中，该酶的最适pH向碱性区域偏移，反应的最大速率降低，而米氏常数保持不变。
