Chadwick E G, Clarkin O M, Raghavendra R, Tanner D A
Materials and Surface Science Institute, University of Limerick, Limerick, Ireland Department of Design and Manufacturing Technology, University of Limerick, Limerick, Ireland.
School of Mechanical and Manufacturing Engineering, Dublin City University, Dublin, Ireland.
Biomed Mater Eng. 2014;24(3):1563-74. doi: 10.3233/BME-140961.
The properties of porous silicon make it a promising material for a host of applications including drug delivery, molecular and cell-based biosensing, and tissue engineering. Porous silicon has previously shown its potential for the controlled release of pharmacological agents and in assisting bone healing. Hydroxyapatite, the principle constituent of bone, allows osteointegration in vivo, due to its chemical and physical similarities to bone. Synthetic hydroxyapatite is currently applied as a surface coating to medical devices and prosthetics, encouraging bone in-growth at their surface and improving osseointegration. This paper examines the potential for the use of an economically produced porous silicon particulate-polytetrafluoroethylene sheet for use as a guided bone regeneration device in periodontal and orthopaedic applications. The particulate sheet is comprised of a series of microparticles in a polytetrafluoroethylene matrix and is shown to produce a stable hydroxyapatite on its surface under simulated physiological conditions. The microstructure of the material is examined both before and after simulated body fluid experiments for a period of 1, 7, 14 and 30 days using Scanning Electron Microscopy. The composition is examined using a combination of Energy Dispersive X-ray Spectroscopy, Thin film X-ray diffraction, Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy and the uptake/release of constituents at the fluid-solid interface is explored using Inductively Coupled Plasma-Optical Emission Spectroscopy. Microstructural and compositional analysis reveals progressive growth of crystalline, 'bone-like' apatite on the surface of the material, indicating the likelihood of close bony apposition in vivo.
多孔硅的特性使其成为一种有前途的材料，可用于许多应用，包括药物递送、基于分子和细胞的生物传感以及组织工程。多孔硅此前已显示出其在控制药物释放和协助骨愈合方面的潜力。羟基磷灰石是骨骼的主要成分，由于其与骨骼在化学和物理性质上的相似性，能够在体内实现骨整合。目前，合成羟基磷灰石被用作医疗设备和假肢的表面涂层，促进其表面的骨向内生长并改善骨整合。本文研究了一种经济生产的多孔硅颗粒-聚四氟乙烯片材用作牙周和骨科应用中引导性骨再生装置的潜力。颗粒片材由聚四氟乙烯基质中的一系列微粒组成，并且在模拟生理条件下其表面会生成稳定的羟基磷灰石。使用扫描电子显微镜对模拟体液实验进行1、7、14和30天前后的材料微观结构进行了检查。使用能量色散X射线光谱、薄膜X射线衍射、衰减全反射-傅里叶变换红外光谱相结合的方法对成分进行了检查，并使用电感耦合等离子体发射光谱法探索了流体-固体界面处成分的吸收/释放情况。微观结构和成分分析表明，材料表面有结晶状“骨样”磷灰石的逐渐生长，这表明在体内可能会有紧密的骨附着。
