Wójtowicz Joanna, Leszczyńska Joanna, Chróścicka Anna, Slósarczyk Anna, Paszkiewicz Zofia, Zima Aneta, Rożniatowski Krzysztof, Jeleń Piotr, Lewandowska-Szumieł Małgorzata
Department of Histology and Embryology, Medical University of Warsaw, Warsaw, Poland.
AGH - Faculty of Materials Science and Ceramics, University of Science and Technology, Cracow, Poland.
Biomed Mater Eng. 2014;24(3):1609-23. doi: 10.3233/BME-140965.
Calcium phosphate ceramics have been widely considered as scaffolds for bone tissue engineering. Selection of the best support for cultured cells, crucial for tissue engineered systems, is still required.
We examined three types of calcium phosphate compounds: α-tricalcium phosphate - the most soluble one, carbonate hydroxyapatite - chemically the most similar to the bone mineral and biphasic calcium phosphate - with the best in vivo biocompatibility in order to select the best support for osteoblastic cells for tissue engineered systems.
Human osteoblasts were tested in direct contact with both dense samples and 3D scaffolds in either static or dynamic culture. Cell viability, cell spreading, osteogenic cell capacity, and extracellular matrix production were examined.
The obtained data indicate that biphasic calcium phosphate is the optimal cell-supporting material. In addition, dynamic culture improved cell distribution in the scaffolds, enhanced production of the extracellular matrix and promoted cells osteogenic capacity.
Biphasic calcium phosphate should be recommended as the most suitable matrix for osteogenic cells expansion and differentiation in tissue engineered systems.
磷酸钙陶瓷已被广泛认为是骨组织工程的支架材料。对于组织工程系统而言，仍需要选择对培养细胞的最佳支持物，这对该系统至关重要。
我们研究了三种磷酸钙化合物：α-磷酸三钙（最易溶解的一种）、碳酸羟基磷灰石（化学组成上与骨矿物质最相似）和双相磷酸钙（体内生物相容性最佳），以便为组织工程系统选择对成骨细胞的最佳支持物。
在静态或动态培养条件下，测试人成骨细胞与致密样品和三维支架的直接接触情况。检测细胞活力、细胞铺展、成骨细胞能力和细胞外基质产生情况。
所获数据表明，双相磷酸钙是最佳的细胞支持材料。此外，动态培养改善了细胞在支架中的分布，增强了细胞外基质的产生，并促进了细胞的成骨能力。
双相磷酸钙应被推荐为组织工程系统中用于成骨细胞扩增和分化的最合适基质。
