[热带假丝酵母饲料酵母的NADP特异性谷氨酸脱氢酶的纯化及性质]
[Purification and properties of the NADP-specific glutamate dehydrogenase of Candida tropicalis feed yeasts].
作者信息
Tret'iakova V A, Inozemtseva I A, Auérman T L
出版信息
Prikl Biokhim Mikrobiol. 1978 Jan-Feb;14(1):18-24.
PMID:24841
Abstract
From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and ion-exchange chromatography on DEAE-cellulose. The preparation was investigated with the aid of polyacrylamide gel electrophoresis. Kinetic characteristics of the enzyme in the cell-free extract and partially purified preparation were derived.
摘要
从饲料酵母热带假丝酵母的无细胞提取物中分离出NADP特异性谷氨酸脱氢酶，并通过硫酸铵分级沉淀和DEAE - 纤维素离子交换色谱法进行了部分纯化（75倍）。借助聚丙烯酰胺凝胶电泳对该制剂进行了研究。得出了无细胞提取物和部分纯化制剂中该酶的动力学特性。
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