Gennari M, Reis M H, Ferreira V C, Siqueira M, Mouton D, Biozzi G
Seçao de Imunologia, Instituto Biologico, São Paulo, Brazil.
Exp Clin Immunogenet. 1986;3(3):172-80.
In order to measure the reciprocal nonspecific effect of the genetic regulation of antibody responsiveness to bovine serum albumin (BSA) and rabbit gamma-globulin (RGG), two independent bidirectional selective breedings for responses to these two antigens were carried out: selection V/BSA and selection V/RGG respectively. The total interline separation at selection limit (RT) was 5.3 log2 for selection V/BSA and 2.6 log2 for selection V/RGG. The sum of these two values (7.9 log2) is similar to the RT in selection V carried out by alternating these two antigens in consecutive generations. In selection V/BSA, the nonspecific effect for responsiveness to RGG was 72%. In selection V/RGG, the nonspecific effect for BSA responsiveness was 135%. The F1 hybrids between homologous lines of selection V/BSA and selection V/RGG presented a larger difference in antibody response to both antigens than their parental lines. This demonstrates an additive effect of the loci controlling the two responses.
为了测量抗体对牛血清白蛋白（BSA）和兔γ球蛋白（RGG）反应的遗传调控的相互非特异性效应，针对这两种抗原的反应进行了两次独立的双向选择性育种：分别为V/BSA选择和V/RGG选择。选择极限时的品系间总分离度（RT），V/BSA选择为5.3 log2，V/RGG选择为2.6 log2。这两个值的总和（7.9 log2）与在连续世代中交替使用这两种抗原进行的V选择中的RT相似。在V/BSA选择中，对RGG反应的非特异性效应为72%。在V/RGG选择中，对BSA反应的非特异性效应为135%。V/BSA选择和V/RGG选择的同源品系之间的F1杂种在对两种抗原的抗体反应上呈现出比其亲本品系更大的差异。这证明了控制这两种反应的基因座的加性效应。
