Shammugasamy Balakrishnan, Ramakrishnan Yogeshini, Ghazali Hasanah M, Muhammad Kharidah
UPM-BERNAS Research Laboratory, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
J Sci Food Agric. 2015 Mar 15;95(4):672-8. doi: 10.1002/jsfa.6742. Epub 2014 Jun 25.
The present study examined the contents of tocopherols and tocotrienols and their distribution in 58 different varieties of whole rice cultivated in Malaysia. The analytical method used was saponification of samples followed by dispersive liquid-liquid microextraction and reverse phase high-performance liquid chromatography.
The total vitamin E contents of different varieties of whole rice ranged between 19.36 and 63.29 mg kg⁻¹. Contents of vitamin E isomers varied among rice varieties both within and between grain color groups. Black-pigmented rice showed significantly higher mean contents of α-tocopherol, β-tocopherol and α-tocotrienol than non-pigmented rice and red-pigmented rice. Red-pigmented rice had significantly lower mean contents of γ-tocotrienol and total vitamin E than non-pigmented rice. The mean contents of δ-tocotrienol and total vitamin E in non-pigmented rice, however, were similar to those in black-pigmented rice. γ-Tocotrienol was the predominant form of vitamin E isomer in all analyzed varieties. The Pearson correlations among vitamin E isomers and total vitamin E content of whole rice were also studied.
This study provides information on vitamin E content of different rice varieties that would be beneficial for decision making in genetic breeding of bioactive compound-rich rice varieties.
本研究检测了马来西亚种植的58个不同品种糙米中生育酚和生育三烯酚的含量及其分布情况。所采用的分析方法是先对样品进行皂化处理,然后进行分散液液微萃取和反相高效液相色谱分析。
不同品种糙米的总维生素E含量在19.36至63.29毫克/千克之间。维生素E异构体的含量在不同水稻品种间以及谷粒颜色组内和组间均有所不同。黑米的α-生育酚、β-生育酚和α-生育三烯酚平均含量显著高于非黑米和红米。红米的γ-生育三烯酚和总维生素E平均含量显著低于非黑米。然而,非黑米中δ-生育三烯酚和总维生素E的平均含量与黑米相似。γ-生育三烯酚是所有分析品种中维生素E异构体的主要形式。同时还研究了糙米中维生素E异构体与总维生素E含量之间的皮尔逊相关性。
本研究提供了不同水稻品种维生素E含量的信息,这将有助于富含生物活性化合物水稻品种的遗传育种决策。