Jinnouchi T, Shibata S, Fukushima M, Mori K
Neurol Med Chir (Tokyo). 1989 Dec;29(12):1065-9. doi: 10.2176/nmc.29.1065.
The microvasculature of ethylnitrosourea (ENU)-induced brain tumors and transplanted 9L cell brain tumors were studied in ultrathin sections and by the freeze fracture replica method. The vessels in ENU-induced tumors were similar to human glioma vessels in that they had endothelial tight junctions and increased pinocytotic vesicles. In the 9L cell tumors, the vessels lacked endothelial tight junctions and had fenestrated endothelium. Macroscopically, Evans blue dye penetrated the 9L cell tumors but not the ENU-induced tumors. Judging from the ultrastructure of the microvessels, the ENU-induced tumor appears more suitable as a human glioma model.
采用超薄切片法和冷冻断裂复型法，对乙基亚硝基脲（ENU）诱导的脑肿瘤和移植的9L细胞脑肿瘤的微血管系统进行了研究。ENU诱导的肿瘤中的血管与人类胶质瘤血管相似，具有内皮紧密连接和增加的胞饮小泡。在9L细胞肿瘤中，血管缺乏内皮紧密连接，内皮有窗孔。宏观上，伊文思蓝染料可穿透9L细胞肿瘤，但不能穿透ENU诱导的肿瘤。从微血管的超微结构判断，ENU诱导的肿瘤似乎更适合作为人类胶质瘤模型。
