Division of Cellular and Molecular Medicine, Department of Physiology and Cell Biology ; Laboratory for Somatic Stem Cell Therapy, Foundation for Biomedical Research and Innovation, Kobe.
Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Kyoto University, Kyoto.
J Diabetes Investig. 2011 Aug 2;2(4):271-5. doi: 10.1111/j.2040-1124.2010.00095.x.
Transplantation of surrogate β-cells is a promising option for the treatment of insulin-deficient diabetes mellitus in the future. Although pancreatic exocrine cells of rodents have been shown to transdifferentiate into insulin-secreting cells, no studies are reported on human exocrine cells. Here, we report the generation of insulin-secreting cells from exocrine cells of the human pancreas. When cultured in suspension with epidermal growth factor, human pancreatic exocrine cells readily formed spherical cell clusters. Expression of Pdx1 was induced in all 19 cases in which we successfully isolated exocrine cells, and insulin expression was induced in 11 cases. In addition, insulin secretion was evaluated in four cases, and the newly-made cells were found to secrete insulin in response to various stimuli. Although further studies are required to improve both the quality and quantity of such insulin-secreting cells, our data suggest that pancreatic exocrine cells represent a potential source of insulin-secreting cells for treatment of type 1 diabetes. (J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00095.x, 2011).
胰岛细胞移植是未来治疗胰岛素缺乏型糖尿病的一种很有前途的选择。虽然啮齿动物的胰腺外分泌细胞已被证明可以转分化为胰岛素分泌细胞，但尚未有关于人外分泌细胞的研究报道。在此，我们报告了从人胰腺外分泌细胞中产生胰岛素分泌细胞的方法。当用人表皮生长因子在悬浮培养中培养时，人胰腺外分泌细胞很容易形成球形细胞簇。我们成功分离出外分泌细胞的 19 例中，所有的 Pdx1 表达均被诱导，11 例中诱导了胰岛素表达。此外，我们对 4 例进行了胰岛素分泌的评估，发现新生成的细胞可以响应各种刺激分泌胰岛素。虽然需要进一步研究来提高这些胰岛素分泌细胞的质量和数量，但我们的数据表明，胰腺外分泌细胞可能是治疗 1 型糖尿病的胰岛素分泌细胞的潜在来源。(J Diabetes Invest, doi: 10.1111/j.2040-1124.2010.00095.x, 2011)。
