Department of Medicine, Metabolism and Endocrinology.
Department of Medicine, Metabolism and Endocrinology ; Sportology Center, Graduate School of Medicine, Juntendo University, Tokyo.
J Diabetes Investig. 2011 Aug 2;2(4):310-7. doi: 10.1111/j.2040-1124.2010.00091.x.
Aims/Introduction: Accumulation of intramyocellular lipid (IMCL) is associated with insulin resistance. However, the factors affecting the change in IMCL remain to be elucidated. The aim of the present study was to determine the factors that influence the change in IMCL level after high-fat loading.
The study subjects were 37 non-obese men. Each subject consumed a high-fat diet for 3 days after a normal-fat diet for 3 days. After each diet program, IMCL levels in the tibialis anterior (TA-IMCL) and soleus (SOL-IMCL) were measured by proton magnetic resonance spectroscopy. Glucose infusion rate (GIR) was evaluated by euglycemic hyperinsulinemic clamp as an index of peripheral insulin sensitivity.
The high-fat diet significantly increased TA-IMCL and SOL-IMCL by ∼30 and ∼20%, respectively (P < 0.05), whereas it did not significantly alter GIR. The increase in SOL-IMCL, but not in TA-IMCL, negatively correlated with serum high molecular weight (HMW)-adiponectin (r = -0.36, P < 0.05) and HMW-/total-adiponectin ratio (r = -0.46, P < 0.05). Although high-fat diet-related changes in SOL-IMCL showed high inter-individual variations, in subjects doing exercise, changes in SOL-IMCL (r = 0.55, P < 0.05) and TA-IMCL (r = 0.61, P < 0.05) positively correlated with daily physical activity level. In contrast, in sedentary subjects, changes in SOL-IMCL (r = -0.50, P < 0.01) and TA-IMCL (r = -0.48, P < 0.05) negatively correlated with daily physical activity.
HMW-adiponectin and daily physical activity are determinants of IMCL accumulation by a high-fat diet. Intriguingly, the effect of daily physical activity on the change in IMCL depends on the level of regular exercise. (J Diabetes Invest,doi: 10.1111/j.2040-1124.2010.00091.x, 2011).
目的/引言：肌内脂质（IMCL）的积累与胰岛素抵抗有关。然而，影响 IMCL 变化的因素仍有待阐明。本研究的目的是确定影响高脂肪负荷后 IMCL 水平变化的因素。
研究对象为 37 名非肥胖男性。每位受试者在正常脂肪饮食 3 天后连续 3 天摄入高脂肪饮食。在每种饮食方案后，通过质子磁共振波谱法测量胫骨前肌（TA-IMCL）和比目鱼肌（SOL-IMCL）中的 IMCL 水平。葡萄糖输注率（GIR）通过正葡萄糖高胰岛素钳夹评估作为外周胰岛素敏感性的指标。
高脂肪饮食显著增加了 TA-IMCL 和 SOL-IMCL，分别增加了约 30%和 20%（P < 0.05），而 GIR 没有显著改变。SOL-IMCL 的增加，但不是 TA-IMCL，与血清高分子量（HMW）-脂联素（r = -0.36，P < 0.05）和 HMW/总脂联素比值（r = -0.46，P < 0.05）呈负相关。尽管高脂肪饮食相关的 SOL-IMCL 变化具有很大的个体间差异，但在进行运动的受试者中，SOL-IMCL 的变化（r = 0.55，P < 0.05）和 TA-IMCL 的变化（r = 0.61，P < 0.05）与每日体力活动水平呈正相关。相比之下，在久坐不动的受试者中，SOL-IMCL 的变化（r = -0.50，P < 0.01）和 TA-IMCL 的变化（r = -0.48，P < 0.05）与每日体力活动呈负相关。
高分子量脂联素和日常体力活动是高脂肪饮食引起 IMCL 积累的决定因素。有趣的是，日常体力活动对 IMCL 变化的影响取决于定期运动的水平。（糖尿病研究杂志，doi: 10.1111/j.2040-1124.2010.00091.x，2011 年）。
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