从新泽西州白纹伊蚊（双翅目：蚊科）中分离出卡谷病毒及其作为区域性虫媒病毒载体的作用评估。

Isolations of Cache Valley virus from Aedes albopictus (Diptera: Culicidae) in New Jersey and evaluation of its role as a regional arbovirus vector.

出版信息

J Med Entomol. 2013 Nov;50(6):1310-4. doi: 10.1603/me13099.

Abstract

The Asian tiger mosquito, Aedes albopictus (Skuse), is an invasive species and a major pest problem in urban and suburban locales in New Jersey. To assess its potential role as an arbovirus vector, we sampled Ae. albopictus from two New Jersey counties over a 3-yr period and estimated the prevalence of virus infection by Vero cell culture and reverse transcription-polymerase chain reaction assays. Three virus isolates were obtained from 34,567 field-collected Ae. albopictus, and all were identified as Cache Valley virus by molecular methods. Ae. albopictus (N = 3,138), collected in Mercer County from late July through early September 2011, also were retested for West Nile virus (WNV) by reverse transcription-polymerase chain reaction, and all were negative. These results corroborate previous findings showing that Ae. albopictus may occasionally acquire Cache Valley virus, a deer-associated arbovirus, in nature. In contrast, we did not detect WNV infection in Ae. albopictus despite concurrent WNV amplification in this region.

摘要

亚洲虎蚊，白纹伊蚊（Skuse），是一种入侵物种，也是新泽西州城市和郊区的主要害虫问题。为了评估其作为虫媒病毒载体的潜在作用，我们在三年内从新泽西州的两个县采集了白纹伊蚊样本，并通过vero 细胞培养和逆转录聚合酶链反应（rt-PCR）检测来估计病毒感染的流行率。从 34567 只野外采集的白纹伊蚊中获得了 3 个病毒分离株，所有分离株均通过分子方法鉴定为卡什谷病毒。2011 年 7 月下旬至 9 月初，在默瑟县采集的白纹伊蚊（n=3138）也通过 rt-PCR 对西尼罗河病毒（WNV）进行了重新检测，结果均为阴性。这些结果证实了之前的研究结果，表明白纹伊蚊在自然界中偶尔会获得与鹿有关的虫媒病毒卡什谷病毒。相比之下，尽管该地区同时存在 WNV 扩增，但我们没有在白纹伊蚊中检测到 WNV 感染。