马克斯克鲁维酵母和克鲁维假丝酵母混合培养用于从乳清中生产单细胞蛋白和去除有机负荷。
Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey.
机构信息
Université du Québec, Institut National de la Recherche Scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec (QC) G1K 9A9, Canada.
Université du Québec, Institut National de la Recherche Scientifique, Centre Eau, Terre & Environnement, 490 de la Couronne, Québec (QC) G1K 9A9, Canada.
出版信息
Bioresour Technol. 2014 Jul;164:119-27. doi: 10.1016/j.biortech.2014.04.069. Epub 2014 May 5.
The study was conducted to evaluate the potential of mixed culture of Kluyveromyces marxianus and Candida krusei to enhance COD removal efficiency, minimize contamination at extreme conditions (high temperature 40°C and low pH 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (SCP) using whey as substrate. The batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher COD removal efficacy with 19% higher biomass yield and 33% increased productivity. The maximum COD removal 80.2% (including residual protein) was obtained at 24h HRT with biomass productivity of 0.17 g/L/h; however, maximum biomass productivity of 0.38 g/L/h and 34% COD removal were obtained at 6h HRT. The results showed that the mixed culture of acid resistance and thermo-tolerant yeasts was a potential way to produce SCP (animal feed) and simultaneous COD removal under extreme operating conditions.
该研究旨在评估马克斯克鲁维酵母（Kluyveromyces marxianus）和克鲁维假丝酵母（Candida krusei）混合培养物在提高 COD 去除效率、最小化高温（40°C）和低 pH（3.5）极端条件下分批和连续好氧发酵过程中的污染以及利用乳清作为底物获得改良品质单细胞蛋白（SCP）方面的潜力。单培养和混合培养的分批发酵结果表明，混合培养可使 COD 去除效率提高 8.8%，生物量产量提高 19%，生产力提高 33%。在 24h HRT 下可获得最大 COD 去除率 80.2%（包括残留蛋白），生物量生产力为 0.17 g/L/h；然而，在 6h HRT 下可获得最大生物量生产力 0.38 g/L/h 和 34%的 COD 去除率。结果表明，耐酸和耐热酵母的混合培养是在极端操作条件下生产 SCP（动物饲料）和同时去除 COD 的一种潜在方法。
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