Yu S Y, Ao P, Wang L M, Huang S L, Chen H C, Lu X P, Liu Q Y
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Biol Trace Elem Res. 1988 Jan-Apr;15:243-55. doi: 10.1007/BF02990141.
Aberrant differentiation is a frequent hallmark of tumors, suggesting that modulators for differentiation and proliferation play a role in multistage carcinogenesis and that their use can also be exploited in cancer chemoprevention and therapy. We have demonstrated that selenium (Se) may be a modulator for the differentiation and proliferation of tumor cells. Evidence has been obtained that Se exerts the following effects: reversing changes of biochemical phenotypes toward normal levels, including reduction of cGMP level and cAMP-dependent protein kinase isozyme type I; increase in cAMP level and cAMP-dependent protein kinase isozyme type II, and altering membrane properties. Furthermore, we have obtained support for this hypothesis utilizing experiments on cultured human liver cell lines. It is demonstrated that Se can lead to the following changes: a. reduction of mitotic index; b. increase in the adhesiveness of cells; c. decrease in confluent saturation density and induction of an early contact inhibition; and d. decrease in tumorigenicity. For the purpose of comparison, the effects of Se on the normal counterparts was also studied. Contrary to what was observed above, there was no significant change in both biochemical and cellular aspects of normal cells treated analogously.
异常分化是肿瘤的常见特征，这表明分化和增殖的调节因子在多阶段致癌过程中发挥作用，并且它们的应用也可用于癌症的化学预防和治疗。我们已经证明，硒（Se）可能是肿瘤细胞分化和增殖的调节因子。已有证据表明硒具有以下作用：将生化表型的变化逆转至正常水平，包括降低cGMP水平和I型cAMP依赖性蛋白激酶同工酶；提高cAMP水平和II型cAMP依赖性蛋白激酶同工酶，并改变膜特性。此外，我们利用对培养的人肝癌细胞系的实验为这一假设提供了支持。结果表明，硒可导致以下变化：a. 降低有丝分裂指数；b. 增加细胞黏附性；c. 降低汇合饱和密度并诱导早期接触抑制；d. 降低致瘤性。为了进行比较，还研究了硒对正常细胞对应物的影响。与上述观察结果相反，类似处理的正常细胞在生化和细胞方面均未出现显著变化。
