Bae Yong Jin, Park Kyung Man, Ahn Sung Hee, Moon Jeong Hee, Kim Myung Soo
Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
J Am Soc Mass Spectrom. 2014 Aug;25(8):1502-5. doi: 10.1007/s13361-014-0919-7. Epub 2014 May 21.
Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.
此前，我们报道过，当温度保持恒定时，肽的基质辅助激光解吸电离（MALDI）光谱变得可重复。从这类光谱数据得出的线性校准曲线可用于定量分析。样品的均匀性是其中一项要求。在肽MALDI中使用的三种常见基质[即α-氰基-4-羟基肉桂酸（CHCA）、2,5-二羟基苯甲酸（DHB）和芥子酸（SA）]中，仅CHCA可通过常规方法制备均匀样品。在本研究中，我们表明通过微点样进行样品制备可改善所有三种情况下的均匀性。
