Whisner Corrie M, Martin Berdine R, Nakatsu Cindy H, McCabe George P, McCabe Linda D, Peacock Munro, Weaver Connie M
Department of Nutrition Science,Purdue University,700 West State Street,West Lafayette,IN47907,USA.
Department of Agronomy,Purdue University,700 West State Street,West Lafayette,IN47907,USA.
Br J Nutr. 2014 Aug 14;112(3):446-56. doi: 10.1017/S0007114514000981. Epub 2014 May 22.
Soluble maize fibre (SCF) has been found to significantly improve bone mineral density and strength in growing rats compared with several other novel prebiotic fibres. The objective of the present study was to investigate the effect of SCF on Ca absorption and retention in pubertal children by studying the potential absorption mechanisms of the intestinal microbiota. A total of twenty-four adolescent boys and girls (12-15 years) participated in two 3-week metabolic balance studies testing 0 g/d SCF (control (CON) treatment) and 12 g/d SCF (SCF treatment) in a random order by inclusion in a low-Ca diet (600 mg/d). Fractional Ca absorption was measured at the end of the two intervention periods using a dual-stable isotope method. Diet composites and faecal and urine samples were collected daily and analysed for Ca content. Ca retention was calculated as dietary Ca intake minus Ca excretion in faeces and urine over the last 2 weeks. Microbial community composition in the faecal samples collected at the beginning and end of each session was determined by 454 pyrosequencing of the PCR-amplified 16S ribosomal RNA gene. Fractional Ca absorption was 12 % higher (41 mg/d) after the SCF treatment compared with that after the CON treatment (0·664 (sd 0·129) and 0·595 (sd 0·142), respectively; P= 0·02), but Ca retention was unaffected. The average proportion of bacteria of the phylum Bacteroidetes was significantly greater in the participants after the SCF treatment than after the CON treatment. These results suggest that moderate daily intake of SCF, a well-tolerated prebiotic fibre, increases short-term Ca absorption in adolescents consuming less than the recommended amounts of Ca.
与其他几种新型益生元纤维相比，已发现可溶性玉米纤维（SCF）能显著提高生长中大鼠的骨矿物质密度和强度。本研究的目的是通过研究肠道微生物群的潜在吸收机制，探讨SCF对青春期儿童钙吸收和潴留的影响。共有24名青少年男孩和女孩（12 - 15岁）参与了两项为期3周的代谢平衡研究，通过纳入低钙饮食（600毫克/天），以随机顺序测试0克/天的SCF（对照（CON）处理）和12克/天的SCF（SCF处理）。在两个干预期结束时，使用双稳定同位素方法测量钙的分数吸收。每天收集饮食复合物以及粪便和尿液样本，并分析其中的钙含量。钙潴留量的计算方法是，用过去两周的膳食钙摄入量减去粪便和尿液中的钙排泄量。通过对PCR扩增的16S核糖体RNA基因进行454焦磷酸测序，确定每次试验开始和结束时收集的粪便样本中的微生物群落组成。与CON处理后相比，SCF处理后的钙分数吸收高出12%（41毫克/天）（分别为0·664（标准差0·129）和0·595（标准差0·142）；P = 0·02），但钙潴留不受影响。SCF处理后的参与者中，拟杆菌门细菌的平均比例显著高于CON处理后。这些结果表明，对于钙摄入量低于推荐量的青少年，适度每日摄入耐受性良好的益生元纤维SCF可增加短期钙吸收。
