伊朗散发性乳腺癌患者中DNA甲基转移酶-3B启动子变异基因型的研究
The Study of DNA Methyltransferase-3B Promoter Variant Genotype among Iranian Sporadic Breast Cancer Patients.
作者信息
Eftekhar Ebrahim, Rasti Mozhgan, Nahgibalhossaini Fakhraddin, Sadeghi Yasaman
机构信息
Deparment of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; ; Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; ; Department of Biochemistry, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Deparment of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
出版信息
Iran J Med Sci. 2014 May;39(3):268-74.
BACKGROUND
DNA methyltransferase-3B (DNMT3B) is an important enzyme responsible for maintaining the DNA methylation pattern in eukaryotic cells. In this study we have investigated the correlation between the 46359C→T polymorphism in the DNMT3B gene and the risk of breast cancer incidence among sporadic breast cancer patients in Fars Province, Southern Iran.
METHODS
In this case-control study, 100 breast cancer patients and 138 healthy control subjects were genotyped for the DNMT3B gene by the polymerase chain reaction-restriction fragment length polymorphism method.
RESULTS
The genotype frequency in the case (CC 27%, CT 47%, TT 26%) group significantly (P=0.008) differed from the control (CC 19.56%, CT 67.3%, TT 13%) group. We observed a decreased association between the CT genotype and lymph node involvement in breast cancer patients. Our results have shown that in comparison to the homozygous CC genotype carriers the DNMT3B-CT genotype has a significantly lower risk for breast cancer (OR=0.515, 95% CI=0.267-0.994, P=0.048).
CONCLUSION
Our case-control study showed that the CT genotype was significantly associated with decreased breast cancer risk. Consistent with these results, a significant decrease of CT genotype among lymph node positive breast cancer patients was observed. However, a larger study population with more clinical data is needed to confirm these results.
背景
DNA甲基转移酶-3B（DNMT3B）是一种负责维持真核细胞中DNA甲基化模式的重要酶。在本研究中，我们调查了伊朗南部法尔斯省散发性乳腺癌患者中DNMT3B基因46359C→T多态性与乳腺癌发病风险之间的相关性。
方法
在这项病例对照研究中，采用聚合酶链反应-限制性片段长度多态性方法对100例乳腺癌患者和138例健康对照者的DNMT3B基因进行基因分型。
结果
病例组（CC 27%，CT 47%，TT 26%）的基因型频率与对照组（CC 19.56%，CT 67.3%，TT 13%）显著不同（P = 0.008）。我们观察到乳腺癌患者中CT基因型与淋巴结受累之间的关联降低。我们的结果表明，与纯合CC基因型携带者相比，DNMT3B-CT基因型患乳腺癌的风险显著更低（OR = 0.515，95% CI = 0.267 - 0.994，P = 0.048）。
结论
我们的病例对照研究表明，CT基因型与降低的乳腺癌风险显著相关。与这些结果一致，在淋巴结阳性乳腺癌患者中观察到CT基因型显著减少。然而，需要更大的研究人群和更多的临床数据来证实这些结果。
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