Mandal Raju Kumar, Haque Shafiul, Wahid Mohd, Jawed Arshad, Akhter Naseem, Khan Md Ekhlaque Ahmed, Panda Aditya Kumar, Areeshi Mohammed Yahya, Dar Sajad Ahmad
Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia.
Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan-45142, Kingdom of Saudi Arabia;; Department of Biosciences, Jamia Millia Islamia (A Central University), New Delhi-110025, India.
Curr Genomics. 2016 Dec;17(6):528-537. doi: 10.2174/1389202917666160530150036.
DNA methyltransferase-3B (DNMT3B) plays a key role in establishment and maintenance of genomic methylation patterns. Polymorphism in promoter region -149 C>T (C46359T) of DNMT3B gene may alter DNMT3B activity which leads to increased susceptibility to cancer. Inconsistent results regarding this have been reported in a number of studies.
To carry out a meta-analysis of the studies reported to assess the precise relationship between the DNMT3B -149 C>T polymorphism and the overall cancer risk.
PubMed (MEDLINE) web database was searched for the studies concerning DNMT3B -149 C>T polymorphism and its association with cancer risk. The pooled odds ratios (ORs) along with 95% confidence intervals (95% CIs) were calculated for all the genetic models, from the selected case-control studies, by meta-analysis.
Overall eighteen studies containing 5583 cancer cases and 7618 controls were analyzed. No significant risk was observed overall for T allele carrier (T vs. C: p=0.303; OR=1.032, 95% CI=0.972-1.097), homozygous (TT vs. CC: p=0.336; OR=1.063, 95% CI=0.939-1.204), heterozygous (CT vs. CC: p=0.802; OR=1.022, 95% CI=0.860-1.216), dominant (TT vs. CC+CT: p=0.298; OR=1.101, 95% CI=0.919-1.319) and recessive (TT+CT vs. CC: p=0.656; OR=1.021, 95% CI=0.931-1.121) genetic models. Subgroup analysis of Asian and Caucasian populations also did not demonstrate any cancer risk in all the genetic models studied.
Our meta-analysis proposes that the DNMT3B -149 C>T polymorphism may not be an independent predisposing factor for the risk of cancer. However, larger sample size and expression studies are required to confirm the observation.
DNA甲基转移酶3B（DNMT3B）在基因组甲基化模式的建立和维持中起关键作用。DNMT3B基因启动子区域-149 C>T（C46359T）多态性可能会改变DNMT3B活性，进而导致患癌易感性增加。多项研究报告了关于此的不一致结果。
对已报道的研究进行荟萃分析，以评估DNMT3B -149 C>T多态性与总体癌症风险之间的确切关系。
在PubMed（MEDLINE）网络数据库中搜索关于DNMT3B -149 C>T多态性及其与癌症风险关联的研究。通过荟萃分析，为所有遗传模型计算选定病例对照研究的合并比值比（OR）及95%置信区间（95%CI）。
共分析了18项研究，包含5583例癌症病例和7618例对照。在所有研究的遗传模型中，总体未观察到T等位基因携带者（T对C：p = 0.303；OR = 1.032，95%CI = 0.972 - 1.097）、纯合子（TT对CC：p = 0.336；OR = 1.063，95%CI = 0.939 - 1.204）、杂合子（CT对CC：p = 0.802；OR = 1.022，95%CI = 0.860 - 1.216）、显性（TT对CC + CT：p = 0.298；OR = 1.101，95%CI = 0.919 - 1.319）和隐性（TT + CT对CC：p = 0.656；OR = 1.021，95%CI = 0.931 - 1.121）遗传模型存在显著风险。对亚洲和白种人群的亚组分析在所有研究的遗传模型中也未显示出任何癌症风险。
我们的荟萃分析表明，DNMT3B -149 C>T多态性可能不是癌症风险的独立易感因素。然而，需要更大样本量和表达研究来证实这一观察结果。
