Turgut Burak, Eren Kenan, Akın Mehmet Mustafa, Demir Tamer, Kobat Sabiha
Department of Ophthalmology, School of Medicine, Fırat University, Elazığ, Turkey.
Department of Pathology, School of Medicine, Fırat University, Elazığ, Turkey.
Drug Des Devel Ther. 2014 May 2;8:421-9. doi: 10.2147/DDDT.S63320. eCollection 2014.
The purpose of this work was to look into the effects of infliximab on wound healing in experimental glaucoma filtration surgery and to compare the antifibrotic effects of this agent to that of mitomycin-C (MMC).
Twenty-eight male New Zealand White rabbits were randomly assigned to four groups, each including seven rabbits: control group, sham group, MMC group, and infliximab group. The rabbits in the control group were not operated on and did not receive any treatment. The rabbits in the sham group underwent trabeculectomy and had one drop of saline instilled four times a day for 14 days. The rabbits in the MMC treatment group underwent trabeculectomy, and a sponge soaked in 0.4 mg/mL MMC was applied intraoperatively to the scleral surgical site for three minutes. The rabbits in the infliximab treatment group underwent trabeculectomy and one drop of 10 mg/mL infliximab was instilled four times a day for 14 days after surgery. On day 14 of the experiment, the operated and control eyes were enucleated and histologically and immunohistochemically analyzed.
The mean fibroblast and mononuclear cell (MNC) numbers and the mean immunostaining intensities of transforming growth factor-β (TGF-β), fibroblast growth factor-β (FGF-β), and platelet-derived growth factor (PDGF) in the sham group were higher than those of the control group (P<0.01). The mean fibroblast and MNC numbers and the mean immunostaining intensities of TGF-β, FGF-β, and PDGF in the MMC and infliximab groups were statistically significantly lower than those of the sham group (P<0.01). The mean fibroblast and MNC numbers and the mean TGF-β, FGF-β, and PDGF immunostaining intensities of the MMC and infliximab groups were similar (P>0.05).
Our study suggests that topical infliximab effectively suppresses the subconjunctival wound healing response after experimental glaucoma filtration surgery, reducing the MNC and fibroblast numbers and immunostaining intensities of TGF-β, FGF-β, and PDGF.
本研究旨在探讨英夫利昔单抗对实验性青光眼滤过手术伤口愈合的影响，并将该药物的抗纤维化作用与丝裂霉素-C（MMC）进行比较。
28只雄性新西兰白兔被随机分为四组，每组7只：对照组、假手术组、MMC组和英夫利昔单抗组。对照组兔子不进行手术，也不接受任何治疗。假手术组兔子接受小梁切除术，每天滴注一滴生理盐水，共4次，持续14天。MMC治疗组兔子接受小梁切除术，术中将浸泡在0.4mg/mL MMC中的海绵应用于巩膜手术部位3分钟。英夫利昔单抗治疗组兔子接受小梁切除术，术后每天滴注一滴10mg/mL英夫利昔单抗，共4次，持续14天。在实验的第14天，摘除手术眼和对照眼，进行组织学和免疫组织化学分析。
假手术组的成纤维细胞和单核细胞（MNC）平均数量以及转化生长因子-β（TGF-β）、成纤维细胞生长因子-β（FGF-β）和血小板衍生生长因子（PDGF）的平均免疫染色强度高于对照组（P<0.01）。MMC组和英夫利昔单抗组的成纤维细胞和MNC平均数量以及TGF-β、FGF-β和PDGF的平均免疫染色强度均显著低于假手术组（P<0.01）。MMC组和英夫利昔单抗组的成纤维细胞和MNC平均数量以及TGF-β、FGF-β和PDGF的平均免疫染色强度相似（P>0.05）。
我们的研究表明，局部应用英夫利昔单抗可有效抑制实验性青光眼滤过手术后结膜下伤口愈合反应，减少MNC和成纤维细胞数量以及TGF-β、FGF-β和PDGF的免疫染色强度。
