Kasar K, Demir T, Akin M M, Gungor Kobat S
Department of Ophthalmology, Ordu State Hospital, Ordu, Turkey.
Department of Ophthalmology, Canakkale Onsekiz Mart University Faculty of Medicine, Canakkale, Turkey.
J Fr Ophtalmol. 2021 Mar;44(3):340-349. doi: 10.1016/j.jfo.2020.04.067. Epub 2021 Jan 22.
To investigate the effects of halofuginone and pirfenidone on wound healing in experimental glaucoma filtration surgery (GFS).
Animal experimentation.
A total of 42 male New Zealand albino rabbits were separated into 6 equal groups. A limbal-based trabeculectomy was performed on 5 groups, and Group I (control group) underwent no surgery and received no postoperative medication. For Group II (sham group), 1 drop 0.9% NaCl was instilled qid for 14 days. For Group III, 1% topical corticosteroid (prednisolone acetate) was instilled 1 drop qid for 14 days. For Group IV, 0.4mg/mL mitomycin-C (MMC) was applied intraoperatively to the region of the scleral flap. For Group V, 0.5% pirfenidone was instilled 1 drop qid for 14 days postoperatively. For Group VI, a sponge soaked in 10ng/mL halofuginone was applied to the surgical region for 3 mins. In addition, 1% topical corticosteroid was instilled ×1 drop qid for 14 days postoperatively for Groups IV, V and VI. After 14 days, sections prepared from the bleb regions of the enucleated eyes were evaluated histopathologically and immunohistochemically. Statistical analyses of the study were performed with Kruskal-Wallis variance analysis and the Mann-Whitney U test.
With regard to fibroblasts, suppression of the number of mononuclear cells and immunohistochemical staining intensity of transforming growth factor-b (TGF-β), fibroblast growth factor-b (FGF-β) and platelet derived growth factor (PDGF), the corticosteroid, MMC, pirfenidone and halofuginone groups were seen to exhibit more effect than the sham group (P<0.05). Compared to the pirfenidone and fuginone groups, inhibition of fibroblast and monocyte proliferation was determined to be lower in the MMC group (P<0.05). The intensity of TGF-β and FGF-β staining was seen to be lower in these two treatment groups than in the MMC group (P<0.05).
Halofuginone and pirfenidone may be used as effective alternative agents in delaying wound healing in glaucoma filtration surgery.
研究卤夫酮和吡非尼酮对实验性青光眼滤过手术（GFS）伤口愈合的影响。
动物实验。
将42只雄性新西兰白兔平均分为6组。对5组进行以角膜缘为基底的小梁切除术，第I组（对照组）未手术且术后未用药。第II组（假手术组）每天4次滴入1滴0.9%氯化钠溶液，持续14天。第III组每天4次滴入1滴1%局部用皮质类固醇（醋酸泼尼松龙），持续14天。第IV组术中在巩膜瓣区域应用0.4mg/mL丝裂霉素C（MMC）。第V组术后每天4次滴入1滴0.5%吡非尼酮，持续14天。第VI组将浸泡在10ng/mL卤夫酮中的海绵敷于手术区域3分钟。此外，第IV、V和VI组术后每天4次滴入1滴1%局部用皮质类固醇，持续14天。14天后，对摘除眼球的滤过泡区域制备的切片进行组织病理学和免疫组织化学评估。采用Kruskal-Wallis方差分析和Mann-Whitney U检验进行统计学分析。
在成纤维细胞、单核细胞数量抑制以及转化生长因子-β（TGF-β）、成纤维细胞生长因子-β（FGF-β）和血小板衍生生长因子（PDGF）的免疫组织化学染色强度方面，皮质类固醇、MMC、吡非尼酮和卤夫酮组比假手术组表现出更强的作用（P<0.05）。与吡非尼酮和卤夫酮组相比，MMC组对成纤维细胞和单核细胞增殖的抑制作用较低（P<0.05）。这两个治疗组中TGF-β和FGF-β染色强度低于MMC组（P<0.05）。
卤夫酮和吡非尼酮可作为青光眼滤过手术中延缓伤口愈合的有效替代药物。
