Kamo S, Takeuchi H, Yamada M
Department of Anatomy, School of Medicine, University of Tokushima, Japan.
Histol Histopathol. 1986 Apr;1(2):103-9.
Visible spectra of erythroid cells stained with Wright's method were studied microphotometrically. Most mature cells of vertebrates have the main peak at 520 nm (A 520) and accessory peaks at 410 nm and 600 nm (A 410 and A 600), respectively. To compare the absorption at 520 nm against 410 nm and 600 nm, the relative absorption spectra were determined and effectively shown as percentage ratios (RAs) to the main peak of A 520. The A 520 shifted to the shorter side of the wavelengths in birds and lower vertebrates. When the RA values of 410 and 600 nm were compared, it was observed that RA 410 of immature cells was less than that of mature cells (15%), and that immature cells of the lower RA 410 type were present in peripheral blood, as well as in bone marrow of vertebrates. Thus, photometric analysis of erythroid cells at RA 410 and RA 600 against A 520 seems to provide useful parameters for comparative studies on vertebrate erythroid cells. Six spectral types can be observed in vertebrate erythroid cells by these parameters.
采用显微光度法研究了经瑞氏法染色的红细胞系细胞的可见光谱。大多数脊椎动物的成熟细胞在520纳米（A 520）处有主峰，在410纳米和600纳米（A 410和A 600）处分别有副峰。为了比较520纳米处相对于410纳米和600纳米处的吸光度，测定了相对吸收光谱，并有效地以相对于A 520主峰的百分比比率（RAs）表示。在鸟类和低等脊椎动物中，A 520向波长较短的一侧移动。比较410纳米和600纳米处的RA值时，观察到未成熟细胞的RA 410低于成熟细胞（15%），并且在脊椎动物的外周血和骨髓中都存在RA 410较低类型的未成熟细胞。因此，针对A 520对红细胞系细胞进行RA 410和RA 600的光度分析似乎为脊椎动物红细胞系细胞的比较研究提供了有用的参数。通过这些参数可以在脊椎动物红细胞系细胞中观察到六种光谱类型。
