一种脂质组学方法用于鉴定拟南芥膜脂组成的冷诱导变化。
A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition.
作者信息
Vu Hieu Sy, Shiva Sunitha, Hall Aaron Smalter, Welti Ruth
机构信息
Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Ackert Hall, Manhattan, KS, 66506, USA.
出版信息
Methods Mol Biol. 2014;1166:199-215. doi: 10.1007/978-1-4939-0844-8_15.
Lipidomic analysis using electrospray ionization triple quadrupole mass spectrometry can be employed to monitor lipid changes that occur during cold and freezing stress of plants. Here we describe the analysis of Arabidopsis thaliana polar glycerolipids with normal and oxidized acyl chains, sampled during cold and freezing treatments. Mass spectral data are processed using the online capabilities of LipidomeDB Data Calculation Environment.
使用电喷雾电离三重四极杆质谱法进行脂质组学分析，可用于监测植物在低温和冷冻胁迫期间发生的脂质变化。在此，我们描述了对拟南芥中具有正常和氧化酰基链的极性甘油脂质的分析，这些脂质是在低温和冷冻处理期间采集的。质谱数据使用LipidomeDB数据计算环境的在线功能进行处理。
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