Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS, USA.
Children's Medical Research Institute at University of Texas-Southwestern, Dallas, TX, USA.
Methods Mol Biol. 2020;2156:187-202. doi: 10.1007/978-1-0716-0660-5_14.
Lipid changes that occur in leaves of plants (e.g., Arabidopsis thaliana), during cold and freezing stress can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM). An online tool, LipidomeDB Data Calculation Environment, is employed for mass spectral data processing.
在冷胁迫和冻胁迫过程中,植物(例如拟南芥)叶片中的脂质变化可以用电喷雾电离三重四极杆质谱分析,采用高通量多重反应监测(MRM)。使用在线工具“脂质组数据库数据计算环境”进行质谱数据处理。