Wang Fen, Bu Guangkui, Feng Qian, Liu Zhiying, Xu Canxia, Shen Shourong, Yuan Yi
Department of Gastroenterology, Third Xiangya Hospital, Central South University , Changsha , China.
Scand J Gastroenterol. 2014 Aug;49(8):925-32. doi: 10.3109/00365521.2014.919015. Epub 2014 May 22.
To investigate the expression level of tumor necrosis factor receptor-associated factor 1 (TRAF1) in gastric mucosa tissue in patients infected with Helicobacter pylori (H. pylori) and to analyze the relationship between TRAF1 expression and H. pylori virulence.
Gastric tissue samples were collected from patients with gastritis, atrophic gastritis, intestinal metaplasia with atypical hyperplasia, and gastric cancer. The expression level of TRAF1 in each group was analyzed by real-time polymerase chain reaction (PCR) and Western blot analysis. Virulence genotypes of H. pylori were determined by PCR.
Significant differences in TRAF1 mRNA levels were observed between the gastritis and gastric cancer groups, and the atrophic gastritis and gastric cancer groups (p < 0.05). Moreover, significant differences in TRAF1 protein levels were observed between the gastritis and intestinal metaplasia with atypical hyperplasia groups, between the gastritis and gastric cancer groups, and between the atrophic gastritis and gastric cancer groups (all p < 0.05). The virulence genotypes of cytotoxin-associated gene A (cagA), vacAs1, and vacAm1 were more frequent in the TRAF1 high-level group than in the TRAF1 low-level group (p < 0.05).
Higher TARF1 expression level is associated with infection by CagA(+)/vacAs1(+)/m1(+) virulent H. pylori strains and may promote the proliferation of gastric mucosal cells and induce gastric cancer.
探讨幽门螺杆菌（H. pylori）感染患者胃黏膜组织中肿瘤坏死因子受体相关因子1（TRAF1）的表达水平，并分析TRAF1表达与H. pylori毒力之间的关系。
收集胃炎、萎缩性胃炎、伴不典型增生的肠化生及胃癌患者的胃组织样本。采用实时聚合酶链反应（PCR）和蛋白质免疫印迹分析检测各组中TRAF1的表达水平。通过PCR确定H. pylori的毒力基因型。
胃炎组与胃癌组、萎缩性胃炎组与胃癌组之间TRAF1 mRNA水平存在显著差异（p < 0.05）。此外，胃炎组与伴不典型增生的肠化生组之间、胃炎组与胃癌组之间以及萎缩性胃炎组与胃癌组之间TRAF1蛋白水平均存在显著差异（均p < 0.05）。细胞毒素相关基因A（cagA）、空泡毒素A s1型（vacAs1）和空泡毒素A m1型（vacAm1）的毒力基因型在TRAF1高水平组中比在TRAF1低水平组中更常见（p < 0.05）。
较高的TRAF1表达水平与CagA(+)/vacAs1(+)/m1(+) 毒力H. pylori菌株感染相关，可能促进胃黏膜细胞增殖并诱发胃癌。
