Drobyshevskaia E I, Nedialkov Iu A, Spitsin S V, Makarova V A, Tarasevich I V, Nesterenko V G
Tr Inst Im Pastera. 1989;66:160-5, 175.
A number of hybridomas against different determinants of R. prowazekii were obtained by hybridization of splenocytes from BALB/c mice immunized with whole cells or soluble antigens of the organism. Some of the monoclonal antibodies bound to thermolabile species specific protein of R. prowazekii and did not react with R. mooseri antigens (monoclonal antibodies series B4/4 and A3). Monoclonal antibodies C5/2 and A3/2 reacted with group specific thermostabile antigen which is common for both R. prowazekii and R. mooseri. Monoclonal antibodies against species specific antigen were found to be of Ig G2a subclass. The monoclonal antibodies can be used to identify R. prowazekii and R. mooseri and solve the problem of differentiating Rickettsiae of typhus group.
通过用普氏立克次体全细胞或可溶性抗原免疫的BALB/c小鼠的脾细胞杂交，获得了多种针对普氏立克次体不同决定簇的杂交瘤。一些单克隆抗体与普氏立克次体的热不稳定种特异性蛋白结合，而不与莫氏立克次体抗原发生反应（B4/4和A3系列单克隆抗体）。C5/2和A3/2单克隆抗体与普氏立克次体和莫氏立克次体共有的群特异性耐热抗原发生反应。发现针对种特异性抗原的单克隆抗体属于Ig G2a亚类。这些单克隆抗体可用于鉴定普氏立克次体和莫氏立克次体，并解决斑疹伤寒群立克次体的鉴别问题。
