Fernandes C C L, Feltrin C, Martins L T, Gaudêncio Neto S, Aguiar L H, Silva A M, Oliveira C H A, Silva L M, Silva C M G, Bertolini M, Rondina D
Faculty of Veterinary Medicine, Ceará State University, Fortaleza, Ceará, Brazil.
Molecular and Developmental Biology Laboratory, University of Fortaleza, Fortaleza, Ceará, Brazil.
Theriogenology. 2014 Jul 15;82(2):332-7. doi: 10.1016/j.theriogenology.2014.04.011. Epub 2014 Apr 18.
Lipid-rich and energy-dense diets can have significant effects on the reproductive physiology, including the ovarian function and fertility. The aim of this study was to assess the effect of cashew nut bran supplementation as a lipid source on follicle development, plasma and intrafollicular concentrations of cholesterol, and developmental competence of in vitro-matured goat oocytes. The inclusion of cashew nut bran as 24% of the goats' diet for 28 days increased the percentage and number of degenerated oocytes compared with the control (P < 0.05), and also the plasma cholesterol levels and the proportion of grade IV oocytes compared with all other treatments (P < 0.05). Moreover, a significant reduction was observed in the proportion of viable oocytes compared with the control and in the percentage of grade II oocytes compared with all other treatments (P < 0.05). Oocyte maturation, cleavage, and blastocyst rates after parthenogenetic activation of viable oocytes were not affected by the type of diet. In conclusion, the inclusion of cashew nut bran as 24% of the diet of adult goats for 28 days changed plasma cholesterol levels and reduced the proportion of viable immature oocytes; however, the 12% and 24% diet supplementations with cashew nut bran did not interfere with competence of resulting viable oocytes to reach the metaphase II stage after IVM, and to develop after parthenogenetic activation.
富含脂质和能量密集型饮食会对生殖生理产生重大影响，包括卵巢功能和生育能力。本研究的目的是评估补充腰果麸作为脂质来源对卵泡发育、血浆和卵泡内胆固醇浓度以及体外成熟山羊卵母细胞发育能力的影响。与对照组相比，在山羊日粮中添加24%的腰果麸28天，会增加退化卵母细胞的百分比和数量（P < 0.05），同时与所有其他处理相比，血浆胆固醇水平和IV级卵母细胞的比例也会增加（P < 0.05）。此外，与对照组相比，存活卵母细胞的比例显著降低，与所有其他处理相比，II级卵母细胞的百分比也显著降低（P < 0.05）。存活卵母细胞孤雌激活后的卵母细胞成熟、分裂和囊胚率不受饮食类型的影响。总之，在成年山羊日粮中添加24%的腰果麸28天会改变血浆胆固醇水平，并降低存活未成熟卵母细胞的比例；然而，日粮中添加12%和24%的腰果麸并不影响所得存活卵母细胞在体外成熟后达到中期II期以及孤雌激活后发育的能力。
