Cui Li, Sun E, Zhang Zhenhai, Tan Xiaobin, Xu Fengjuan, Jia Xiaobin
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing, 210028, Jiangsu Province, China.
Biomed Chromatogr. 2014 Oct;28(10):1306-12. doi: 10.1002/bmc.3174. Epub 2014 May 23.
Epimedin C is one of the major bioactive constituents of Herba Epimedii. In this study, the metabolite profiles of epimedin C in rat plasma and bile were qualitatively investigated, and the possible metabolic pathways of epimedin C were subsequently proposed. After oral administration of epimedin C at a single dose of 80 mg/kg, rat biological samples were collected and pretreated by protein precipitation. Then these pretreated samples were injected into an Acquity UPLC BEH C18 column and detected by ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry. In all, 12 metabolites were identified in the biosamples. Of these, eight, two from plasma and six from bile, are, to our knowledge, reported here for the first time. The results indicated that epimedin C was metabolized via desugarization, dehydrogenation, hydrogenation, dehydroxylation, hydroxylation, demethylation and glucuronidation pathways in vivo. Thus, this study revealed the possible metabolite profiles of epimedin C in rat plasma and bile.
淫羊藿苷C是淫羊藿的主要生物活性成分之一。本研究对淫羊藿苷C在大鼠血浆和胆汁中的代谢产物谱进行了定性研究，并随后提出了淫羊藿苷C可能的代谢途径。以80 mg/kg的单剂量口服给予淫羊藿苷C后，收集大鼠生物样品并通过蛋白沉淀法进行预处理。然后将这些预处理后的样品注入Acquity UPLC BEH C18柱，并通过超高效液相色谱/四极杆-飞行时间质谱进行检测。在生物样品中总共鉴定出12种代谢产物。其中，据我们所知，有8种是首次在此报道，2种来自血浆，6种来自胆汁。结果表明，淫羊藿苷C在体内通过去糖基化、脱氢、氢化、脱羟基、羟基化、去甲基化和葡萄糖醛酸化途径进行代谢。因此，本研究揭示了淫羊藿苷C在大鼠血浆和胆汁中可能的代谢产物谱。
