Department of Pathology, The First Affiliated Hospital, Zhejiang University Medical College, Hangzhou, China.
Department of Hepatobiliary Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):1-6. doi: 10.1016/j.bbrc.2014.05.045. Epub 2014 May 20.
eEF1A2 is a protein translation factor involved in protein synthesis, which possesses important function roles in cancer development. This study aims at investigating the expression pattern of eEF1A2 in prostate cancer and its potential role in prostate cancer development.
We examined the expression level of eEF1A2 in 30 pairs of prostate cancer tissues by using RT-PCR and immunohistochemical staining (IHC). Then we applied siRNA specifically targeting eEF1A2 to down-regulate its expression in DU-145 and PC-3 cells. Flow cytometer was used to explore apoptosis and Western-blot was used to detect the pathway proteins of apoptosis.
Our results showed that the expression level of eEF1A2 in prostate cancer tissues was significantly higher compared to their corresponding normal tissues. Reduction of eEF1A2 expression in DU-145 and PC-3 cells led to a dramatic inhibition of proliferation accompanied with enhanced apoptosis rate. Western blot revealed that apoptosis pathway proteins (caspase3, BAD, BAX, PUMA) were significantly up-regulated after suppression of eEF1A2. More importantly, the levels of eEF1A2 and caspase3 were inversely correlated in prostate cancer tissues.
Our data suggests that eEF1A2 plays an important role in prostate cancer development, especially in inhibiting apoptosis. So eEF1A2 might serve as a potential therapeutic target in prostate cancer.
eEF1A2 是一种参与蛋白质合成的蛋白质翻译因子，在癌症发展中具有重要的功能作用。本研究旨在研究 eEF1A2 在前列腺癌中的表达模式及其在前列腺癌发展中的潜在作用。
我们通过 RT-PCR 和免疫组织化学染色（IHC）检测了 30 对前列腺癌组织中 eEF1A2 的表达水平。然后，我们应用特异性靶向 eEF1A2 的 siRNA 下调 DU-145 和 PC-3 细胞中的表达。流式细胞仪用于探索细胞凋亡，Western blot 用于检测凋亡途径蛋白。
我们的结果表明，前列腺癌组织中 eEF1A2 的表达水平明显高于相应的正常组织。eEF1A2 表达降低在 DU-145 和 PC-3 细胞中导致增殖明显抑制，凋亡率增加。Western blot 显示，抑制 eEF1A2 后凋亡途径蛋白（caspase3、BAD、BAX、PUMA）显著上调。更重要的是，前列腺癌组织中 eEF1A2 和 caspase3 的水平呈负相关。
我们的数据表明，eEF1A2 在前列腺癌发展中起重要作用，特别是在抑制细胞凋亡方面。因此，eEF1A2 可能成为前列腺癌的潜在治疗靶点。
