Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University , Harbin , China and.
Biomarkers. 2014 Jun;19(4):275-80. doi: 10.3109/1354750X.2014.904000.
This study aims to test the serum levels of soluble thrombomodulin (TM) in patients with chronic kidney disease (CKD)3-5 and to assess their connection with the different stages and severity of disease.
Sixty-seven patients with CKD are included, disease severity was evaluated accordingly to CKD staging and clinical data is collected. Nineteen healthy volunteers served as healthy controls. Serum soluble TM is analyzed by ELISA.
The levels of soluble TM in all patients with CKD were significantly higher than those of healthy controls (p < 0.001). CKD5 patients showed higher serum levels of soluble TM, in comparison to CKD4 patients (p = 0.001), CKD3 patients (p < 0.001), and healthy controls (p < 0.001). The correlation analysis revealed significant correlation between serum soluble TM and disease severity (r = 0.714, p < 0.001). Serum soluble TM was found to be correlated with eGFR (r = -0.766; p < 0.001) and serum creatinine (r = 0.778, p < 0.001).
Soluble TM concentrations significantly increase in the CKD patients and are associated with the severity of the disease. Soluble TM may play critical roles in the development of CKD, as a biomarker of endothelial cells damage, anticoagulation and anti-inflammation.
本研究旨在检测慢性肾脏病（CKD）3-5 期患者的血清可溶性血栓调节蛋白（TM）水平，并评估其与疾病不同阶段和严重程度的关系。
纳入 67 例 CKD 患者，根据 CKD 分期评估疾病严重程度并收集临床资料。19 名健康志愿者作为健康对照组。采用 ELISA 法分析血清可溶性 TM。
所有 CKD 患者的可溶性 TM 水平均明显高于健康对照组（p<0.001）。CKD5 期患者的血清可溶性 TM 水平高于 CKD4 期患者（p=0.001）、CKD3 期患者（p<0.001）和健康对照组（p<0.001）。相关性分析显示，血清可溶性 TM 与疾病严重程度呈显著正相关（r=0.714，p<0.001）。血清可溶性 TM 与 eGFR（r=-0.766；p<0.001）和血清肌酐（r=0.778，p<0.001）呈显著正相关。
可溶性 TM 浓度在 CKD 患者中显著升高，并与疾病严重程度相关。可溶性 TM 可能在 CKD 的发生发展中起重要作用，是内皮细胞损伤、抗凝和抗炎的生物标志物。
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