Araya Magdalena, Gutiérrez Ricardo, Arredondo Miguel
Institute of Nutrition and Food Technology (INTA), University of Chile, Av. El Líbano, 5524, Macul, Santiago, Chile,
Biometals. 2014 Aug;27(4):645-52. doi: 10.1007/s10534-014-9737-4. Epub 2014 May 23.
The chaperone to Zn-Cu superoxide dismutase (CCS) has been postulated as a candidate copper indicator, changing in a consistent manner in induced and recovered copper deficiency, in experimental cell and animal models. In real life people have various conditions that may modify molecules acting as acute phase proteins, such as serum ceruloplasmin and copper concentration and could alter CCS responses. With the hypothesis that CCS mRNA transcripts and protein would be different in individuals suffering inflammatory processes in comparison to healthy individuals, we assessed adult individuals who, although not ill had conditions known to induce variable degrees of inflammation. Screening of 600 adults resulted in two study groups, formed on the basis of their clinical history and levels of serum C reactive protein (CRP): Group 1 (n = 61, mean (range) CRP = 0.9 (0.3-2.0 mg/dL) and Group 2 (n = 150, mean (range) CRP = 6.1 (4.3-8.7 mg/dL). Results showed that mRNA transcripts relative abundance was not different for CCS, MTIIA, TNF-alpha and Cu-Zn-SOD by group (p > 0.05, one way Anova), nor between sexes (p > 0.05, one way Anova). Distribution of CCS mRNA transcripts and CCS protein in serum did not show any differences or trends. Results disproved our hypothesis that CCS abundance of transcripts and CCS protein would be different in individuals suffering inflammatory processes, adding further support to the idea that CCS may be a copper marker.
锌铜超氧化物歧化酶伴侣蛋白（CCS）被假定为一种候选铜指标，在实验性细胞和动物模型中，其在诱导性和恢复性铜缺乏时会以一致的方式发生变化。在现实生活中，人们有各种可能改变作为急性期蛋白的分子的状况，如血清铜蓝蛋白和铜浓度，并且可能改变CCS反应。基于患有炎症过程的个体与健康个体相比，CCS mRNA转录本和蛋白质会有所不同这一假设，我们评估了一些成年个体，这些个体虽然没有患病，但有已知会诱导不同程度炎症的状况。对600名成年人进行筛查后，根据他们的临床病史和血清C反应蛋白（CRP）水平形成了两个研究组：第1组（n = 61，平均（范围）CRP = 0.9（0.3 - 2.0mg/dL））和第2组（n = 150，平均（范围）CRP = 6.1（4.3 - 8.7mg/dL））。结果显示，CCS、MTIIA、TNF-α和铜锌超氧化物歧化酶的mRNA转录本相对丰度在组间无差异（p > 0.05，单因素方差分析），在性别之间也无差异（p > 0.05，单因素方差分析）。血清中CCS mRNA转录本和CCS蛋白的分布未显示出任何差异或趋势。结果反驳了我们的假设，即患有炎症过程的个体中CCS转录本丰度和CCS蛋白会有所不同，进一步支持了CCS可能是一种铜标志物的观点。
