Poumarat F, Perrin M, Belli P, Martel J L
Laboratoire de Pathologie Bovine, Centre National d'Etudes Vétérinaires et Alimentaires, Lyon, France.
Rev Elev Med Vet Pays Trop. 1989;42(3):357-64.
During an experimental reproduction of CBPP, 5 inoculated cows and 5 contacts cows were bled twice a week and antibodies research was performed using complement fixation test (CFT), passive haemagglutination test (PHAT) and slide agglutination test (SAT). In the same period, trachobronchial washings were performed weekly to detect and to count Mycoplasma mycoides subsp. mycoides SC. For four months these tests were used to compare antibodies kinetics with kinetics of mycoplasmas excretion. With titer over 40 as threshold of positivity, PHAT detects antibodies earlier than CFT and SAT at the beginning of infection, but fails to detect chronic carriers. In contacts cows no failure has been observed with CFT and SAT except during prodromic phase of infection. These results are valid for natural infections. However, for inoculated animals, most serious failures to detect infected cows occur.
在牛传染性胸膜肺炎(CBPP)的实验性复制过程中,每周对5头接种牛和5头接触牛采血两次,并使用补体结合试验(CFT)、被动血凝试验(PHAT)和玻片凝集试验(SAT)进行抗体研究。在同一时期,每周进行气管支气管冲洗以检测和计数丝状支原体丝状亚种SC型(Mycoplasma mycoides subsp. mycoides SC)。在四个月的时间里,这些检测用于比较抗体动力学与支原体排泄动力学。以滴度超过40作为阳性阈值,在感染初期PHAT比CFT和SAT更早检测到抗体,但无法检测出慢性携带者。在接触牛中,除了感染前驱期外,CFT和SAT未出现漏检情况。这些结果适用于自然感染。然而,对于接种动物,在检测感染牛时出现了最严重的漏检情况。