Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
China Military Institute of Chinese Medicine, Integrative Medical Center of 302 Military Hospital, Beijing 100039, China.
Chin J Nat Med. 2014 May;12(5):393-400. doi: 10.1016/S1875-5364(14)60049-2.
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that causes both hospital- and community-acquired infections, and for which single-drug treatments are becoming less efficient. Rhizoma coptidis has been used for more than two thousand years in China to treat diarrhea, fever, and jaundice. In this study, the anti-MRSA activity of Rhizoma coptidis is examined and its effective components sought.
The mecA and norA genes were determined by PCR amplification and sequencing. Drug susceptibility of Staphylococcus aureus ATCC43300 was performed using the VITEK2 compact system. The chemical fingerprint of Rhizoma coptidis was investigated using HPLC and preparative liquid chromatography, and the anti-MRSA activity was determined using an improved broth microdilution method.
The drug susceptibility test revealed that the penicillin-binding protein phenotype of the strain changed in comparison to penicillin-sensitive Staphylococcus aureus. Ten batches of Rhizoma coptidis showed anti-MRSA activity on the norA-negative Staphylococcus aureus strain, as well as the strain that contained a norA gene. The spectrum-effect relationship revealed that the berberine alkaloids were the effective components, within which berberine, coptisine, palmatine, epiberberine, and jatrorrhizine were the major components.
This study lays a foundation for in vivo studies of Rhizoma coptidis and for the development of multi-component drugs.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种致病性细菌，可引起医院获得性和社区获得性感染，并且单一药物治疗的效果越来越差。黄连在中国已经使用了两千多年，用于治疗腹泻、发热和黄疸。在这项研究中，检查了黄连的抗 MRSA 活性并寻找其有效成分。
通过 PCR 扩增和测序确定 mecA 和 norA 基因。使用 VITEK2 compact 系统测定金黄色葡萄球菌 ATCC43300 的药敏性。采用 HPLC 和制备型液相色谱法研究黄连的化学指纹图谱，并采用改良肉汤微量稀释法测定抗 MRSA 活性。
药敏试验显示，与青霉素敏感的金黄色葡萄球菌相比，该菌株的青霉素结合蛋白表型发生了变化。十批黄连对 norA 阴性金黄色葡萄球菌株以及含有 norA 基因的菌株均具有抗 MRSA 活性。谱效关系表明，小檗碱类生物碱是有效成分，其中小檗碱、黄连碱、巴马汀、表小檗碱和药根碱是主要成分。
本研究为黄连的体内研究和多成分药物的开发奠定了基础。
Zotero 插件