Huang Xiao-ping, Li Long-yun, Qu Xian-you, Cui Guang-lin
Chongqing Academy of Chinese Materia Medica, China.
Zhong Yao Cai. 2006 Jul;29(7):666-9.
To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing.
The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm.
The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine.
The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.
建立一种灵敏且特异的高效液相色谱法，用于重庆石柱产黄连的质量控制。
采用沃特世仪器获取石柱黄连的高效液相色谱指纹图谱。该方法在迪马硅基C18柱上进行，以乙腈-0.05mol/L磷酸二氢钾（用磷酸调pH值至3）为流动相进行梯度洗脱，流速为0.8ml/min。柱温为25℃，紫外检测波长为270nm。
从10批黄连中建立了具有16个特征峰的黄连高效液相色谱指纹图谱。通过与化学对照品的保留时间和在线紫外光谱进行比较，峰11、12、13、14和15分别被鉴定为表小檗碱、药根碱、黄连碱、巴马汀和小檗碱。
具有高特异性的黄连高效液相色谱指纹图谱可用于其质量控制。
